Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Binzhou Animal Husbandry and Veterinary Research Academy, Binzhou 256600, People's Republic of China.
Appl Biochem Biotechnol. 2011 Apr;163(7):845-50. doi: 10.1007/s12010-010-9088-8. Epub 2010 Sep 16.
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.
在过去的 10 年中，随着环介导等温扩增（LAMP）方法的发展，由于其简单、快速、高效和出色的特异性，已被广泛应用于核酸分析。该方法使用一种 DNA 聚合酶和一组四个专门设计的引物，总共识别目标 DNA 上的六个不同序列。与常规 PCR 和实时 PCR 检测相比，不需要昂贵的设备就可以获得高精度，而且准备步骤也更少。本文简要总结了 LAMP 方法在病原微生物、转基因成分、肿瘤检测和胚胎性别鉴定中的应用。
