Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Changsari, Kamrup, Guwahati, Assam, India.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Changsari, Kamrup, Guwahati, Assam, India.
J Biochem Mol Toxicol. 2022 Aug;36(8):e23113. doi: 10.1002/jbt.23113. Epub 2022 Jun 1.
An outbreak of the novel beta coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) first came to light in December 2019, which has unfolded rapidly and turned out to be a global pandemic. Early prognosis of viral contamination involves speedy intervention, disorder control, and good-sized management of the spread of disease. Reverse transcription-polymerase chain reaction, considered the gold standard test for detecting nucleic acids and pathogen diagnosis, provides high sensitivity and specificity. However, reliance on high-priced equipped kits, associated reagents, and skilled personnel slow down sickness detection. Lately, the improvement of clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated protein)-based diagnostic systems has reshaped molecular diagnosis due to their low cost, simplicity, speed, efficiency, high sensitivity, specificity, and versatility, which is vital for accomplishing point-of-care diagnostics. We reviewed and summarized CRISPR-Cas-based point-of-care diagnostic strategies and research in these paintings while highlighting their characteristics and challenges for identifying SARS-CoV-2.
一种新型的β冠状病毒——严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）于 2019 年 12 月首次被发现，其迅速蔓延并已成为全球性的大流行。病毒污染的早期预后涉及快速干预、紊乱控制和疾病传播的有效管理。逆转录-聚合酶链反应被认为是检测核酸和病原体诊断的金标准试验，具有高灵敏度和特异性。然而，对昂贵的设备试剂盒、相关试剂和熟练人员的依赖会减缓疾病的检测速度。最近，基于成簇规律间隔短回文重复（CRISPR）-Cas（CRISPR 相关蛋白）的诊断系统的改进，由于其成本低、简单、快速、高效、高灵敏度、特异性和多功能性，重塑了分子诊断，这对于实现即时诊断至关重要。我们综述并总结了基于 CRISPR-Cas 的即时诊断策略和研究进展，同时强调了它们在识别 SARS-CoV-2 方面的特点和挑战。
