Department of Biology, Faculty of Arts and Science, Muş Alparslan University, İstasyon Street, 49100 Muş, Turkey.
Mol Biol Rep. 2011 Mar;38(3):1713-6. doi: 10.1007/s11033-010-0284-y. Epub 2010 Sep 16.
This study was conducted in Turkish osteoarthritis patients to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (135 patients with osteoarthritis and 65 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (P < 0.001). The D allele frequency was indicated as 69% and I allele was as 31% in the patients, whereas it was 55-45% in the control group. Consequently, in this study, we may assert that ACE gene I/D polymorphism DD genotype determination is significant criteria for identifying patients who are likely to develop osteoarthritis in east population of Turkey.
本研究旨在土耳其骨关节炎患者中确定血管紧张素转换酶基因 I/D 多态性基因型的频率，并探讨该多态性在骨关节炎发病机制中的作用。研究中使用了 200 名个体（135 名骨关节炎患者和 65 名健康对照者）的基因组 DNA。使用 I 和 D 等位基因特异性引物通过聚合酶链反应扩增 DNA。通过将聚合酶链反应产物暴露于 2%琼脂糖凝胶电泳，用 CCD 相机进行评估。在基因型分布方面，组间存在统计学差异（P<0.001）。在患者中，D 等位基因频率为 69%，I 等位基因为 31%，而在对照组中为 55-45%。因此，在本研究中，我们可以断言 ACE 基因 I/D 多态性 DD 基因型的确定是识别土耳其东部人群中可能发生骨关节炎的患者的重要标准。
