Gaziosmanpasa University, Faculy of Medicine, Department of Medical Biology, Tokat, Turkey.
Gene. 2012 Dec 10;511(1):106-8. doi: 10.1016/j.gene.2012.09.036. Epub 2012 Sep 20.
We sought to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene in Turkish patients with rheumatoid arthritis. Genomic DNA obtained from 256 individuals (110 patients with rheumatoid arthritis and 146 healthy controls) was used in the study. ACE gene I/D polymorphism genotypes were determined using polymerase chain reaction using I and D allele-specific primers. There was a statistically significant difference between the groups with respect to genotype distribution (p=0.001). A significant difference was found in frequencies of ACE I/D alleles between patients and controls, with RA patients having a higher representation of D and lower representation of I alleles compared to controls (p<0.001). As a result of our study, angiotensin converting enzyme gene I/D polymorphism DD genotype could be a genetic marker in rheumatoid arthritis in the Turkish study population.
我们旨在确定土耳其类风湿关节炎患者血管紧张素转换酶基因 I/D 多态性基因型的频率。该研究使用了从 256 个人（110 例类风湿关节炎患者和 146 例健康对照者）获得的基因组 DNA。使用 I 和 D 等位基因特异性引物通过聚合酶链反应确定 ACE 基因 I/D 多态性基因型。组间基因型分布存在统计学差异（p=0.001）。患者和对照组之间 ACE I/D 等位基因的频率存在显著差异，与对照组相比，RA 患者的 D 等位基因代表率更高，而 I 等位基因的代表率更低（p<0.001）。我们的研究结果表明，血管紧张素转换酶基因 I/D 多态性 DD 基因型可能是土耳其研究人群中类风湿关节炎的遗传标志物。
