Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia.
AAPS PharmSciTech. 2010 Sep;11(3):1485-92. doi: 10.1208/s12249-010-9507-8. Epub 2010 Sep 16.
The aim of this work was to test innovative approach for enhancing ascorbyl palmitate stability in microemulsions for topical application by addition of newly synthesized co-antioxidant 4-(tridecyloxy)benzaldehyde oxime (TDBO) and to investigate its antioxidant activity and finally to evaluate cytotoxicity of TDBO-loaded microemulsions on keratinocyte cells. TDBO significantly increased ascorbyl palmitate stability in oil-dispersed-in-water (o/w) microemulsions, most presumably due to reduction of ascorbyl palmitate radical back to ascorbyl palmitate, since TDBO free-radical scavenging activity was confirmed. Cytotoxicity experiments demonstrated no significant change in cell viability or morphology in the presence of TDBO-loaded microemulsions regarding unloaded microemulsions, although greater cytotoxicity was observed with increased microemulsion concentrations. Therefore, the incorporation of TDBO as non-cytotoxic co-antioxidant in o/w microemulsions is a promising new strategy for enhancing ascorbyl palmitate stability that could be used to support antioxidant network in the skin.
本工作旨在通过添加新合成的共抗氧化剂 4-(十三烷氧基)苯甲醛肟（TDBO）来测试增强用于局部应用的微乳液中抗坏血酸棕榈酸酯稳定性的创新方法，并研究其抗氧化活性，最后评估 TDBO 负载的微乳液对角质形成细胞的细胞毒性。TDBO 显著提高了油分散在水中（o/w）微乳液中抗坏血酸棕榈酸酯的稳定性，最可能是由于抗坏血酸棕榈酸酯自由基被还原回抗坏血酸棕榈酸酯，因为已经证实 TDBO 具有自由基清除活性。细胞毒性实验表明，与未负载的微乳液相比，负载 TDBO 的微乳液对细胞活力或形态没有显著变化，尽管随着微乳液浓度的增加，观察到更大的细胞毒性。因此，将 TDBO 作为非细胞毒性共抗氧化剂掺入 o/w 微乳液中是增强抗坏血酸棕榈酸酯稳定性的一种很有前途的新策略，可用于支持皮肤中的抗氧化网络。
