Ekman S, Heinegård D, Johnell O, Rodriguez-Martinez H
Department of Anatomy and Histology, Swedish University of Agricultural Sciences, Uppsala.
Matrix. 1990 Dec;10(6):402-11. doi: 10.1016/s0934-8832(11)80148-4.
Osteochondrosis is an impaired focal endochondral ossification which appears as a cartilage retention in the subchondral bone of growing pigs. The normal differentiation of chondrocytes does not occur and the matrix calcification is restricted. The present investigation has compared the content of selected macromolecules in the cartilage matrix of the normal articular and epiphyseal growth cartilage with the osteochondrotic cartilage, using a peroxidase-antiperoxidase (PAP) immunocytochemical method at light microscopical level. Some of the non-collagenous macromolecules (fibromodulin, large aggregating proteoglycans, fibronectin, 100-kDa subunit protein and 148-kDa protein) were conspicuously prominent within the osteochondrotic cartilage, compared to the matrix of the "normal" resting, proliferative and hypertrophic regions. This indicates that the chondrocytes in the osteochondrotic cartilage do not modify their surrounding matrix adequately, thus precluding normal calcification.
骨软骨病是一种局限性软骨内成骨受损的疾病，表现为生长猪的软骨下骨中存在软骨滞留。软骨细胞的正常分化未发生，基质钙化受到限制。本研究使用过氧化物酶-抗过氧化物酶（PAP）免疫细胞化学方法，在光学显微镜水平上比较了正常关节和骨骺生长软骨的软骨基质与骨软骨病软骨中选定大分子的含量。与“正常”静止、增殖和肥大区域的基质相比，一些非胶原大分子（纤维调节素、大聚合蛋白聚糖、纤连蛋白、100-kDa亚基蛋白和148-kDa蛋白）在骨软骨病软骨中明显突出。这表明骨软骨病软骨中的软骨细胞不能充分改变其周围基质，从而妨碍了正常钙化。
