Thorp B H, Ekman S, Jakowlew S B, Goddard C
Roslin Institute (Edinburgh), Midlothian, Scotland.
Calcif Tissue Int. 1995 May;56(5):376-81. doi: 10.1007/BF00301606.
Osteochondrosis and dyschondroplasia are common multifocal disturbances of endochondral ossification in many species of domestic animals, and are characterized by the retention of avascular cartilage. These cartilage disorders are characterized by a failure of chondrocyte differentiation, matrix mineralisation and its replacement by bone. Rabbit polyclonal antibodies to transforming growth factor-beta (TGF-beta) and to insulin-like growth factor-I (IGF-I) were used to detect the two growth factors in normal and osteochondrotic porcine epiphyses. In the normal pig epiphyses IGF-I and TGF-beta were present in the chondrocytes of the epiphyseal hyaline cartilage and IGF-I was readily localised to the hypertrophic chondrocytes in the growth cartilage adjacent to the epiphyseal ossification centre. Both growth factors were found to be deficient in chondrocytes at sites of osteochondrosis. Both these growth factors are thought to be involved in the cascade of events associated with chondrocyte function during endochondral ossification. Deficiencies in TGF-beta and IGF-I demonstrated in porcine osteochondrosis and previously shown in avian dyschondroplasia suggest further similarities in the pathogenesis of these conditions.
骨软骨病和软骨发育异常是许多家畜常见的多灶性软骨内成骨紊乱，其特征是无血管软骨的保留。这些软骨疾病的特征是软骨细胞分化、基质矿化以及被骨替代失败。用兔抗转化生长因子-β（TGF-β）和抗胰岛素样生长因子-I（IGF-I）多克隆抗体检测正常和患骨软骨病猪骨骺中的这两种生长因子。在正常猪骨骺中，IGF-I和TGF-β存在于骨骺透明软骨的软骨细胞中，IGF-I很容易定位于骨骺骨化中心附近生长软骨中的肥大软骨细胞。在骨软骨病部位的软骨细胞中发现这两种生长因子都缺乏。这两种生长因子都被认为参与了软骨内成骨过程中与软骨细胞功能相关的一系列事件。猪骨软骨病中显示的TGF-β和IGF-I缺乏以及先前在禽类软骨发育异常中显示的缺乏表明这些病症在发病机制上有进一步的相似性。
