Department of Chemistry, The Skaggs Institute for Chemical Biology and The Worm Institute for Research and Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Angew Chem Int Ed Engl. 2010 Dec 10;49(50):9630-4. doi: 10.1002/anie.201003387.
Assays armed with catalytic signal amplification have arisen as superior systems for ultrasensitive detection of analytes. Here we describe a conceptually new enzyme assay based on cat-ELISA, (cat-ELCCA), where an enzyme-linked azide is utilized to arm the assay with catalytic fluorescence signal amplification. Using this assay technology, we have developed the first potentially high-throughput screen for the recently disclosed acyltransferase, ghrelin -acyltransferase (GOAT).
基于催化信号放大的分析方法已经成为超灵敏分析物检测的优越系统。在这里，我们描述了一种基于 cat-ELISA 的新概念酶分析方法（cat-ELCCA），其中使用酶联叠氮化物为分析方法提供具有催化荧光信号放大的能力。利用这种分析技术，我们开发了首个针对最近发现的酰基转移酶（ghrelin-acyltransferase，GOAT）的高通量筛选方法。
