Reymond Jean-Louis, Fluxà Viviana S, Maillard Noélie
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne, 3012, Switzerland.
Chem Commun (Camb). 2009 Jan 7(1):34-46. doi: 10.1039/b813732c. Epub 2008 Oct 17.
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.
酶活性测定是用于可视化酶活性的分析工具。近年来，已经开发出了各种各样的酶活性测定方法，以协助工业酶的发现和优化，特别是在“白色生物技术”领域，在该领域中，选择性酶被成功用于经济可行、温和且环境友好的生产过程。本文重点介绍了荧光和显色底物、传感器以及酶指纹图谱等方面，这些是我们特别感兴趣的领域。
