Karataev G I, Shumakov Iu L, Kirillov M Iu, Volgina T I, Lapaeva I A
Mol Gen Mikrobiol Virusol. 1990 Dec(12):27-32.
To study the structural arrangement of the chromosomal region containing vir genes of Bordetella pertussis the corresponding 15 kb fragment of Bordetella pertussis chromosomal DNA has been cloned. The sequence homology to an earlier characterized Bordetella pertussis genetical element RSBP1 and flanked by two 400 bp inverted repeats has been shown to be located at an end of a BamHI fragment. The restriction map of Bordetella pertussis 475 coincides with the previously published maps of Bordetella pertussis Tohama and 18323 permitting one to conclude the definite conservatism of the cloned sequence. The preliminary data obtained make possible mapping of the RSBP1 homologous sequence adjacent to adenylate cyclase, agglutinin 2 and pertussis toxin genes. The possible role of RSBP1 elements in the regulation of Bordetella virulence is suggested.
为研究含有百日咳博德特氏菌毒力基因的染色体区域的结构排列，已克隆了百日咳博德特氏菌染色体DNA的相应15kb片段。与先前鉴定的百日咳博德特氏菌遗传元件RSBP1具有序列同源性且两侧有两个400bp反向重复序列的片段，已被证明位于BamHI片段的一端。百日咳博德特氏菌475的限制性图谱与先前发表的百日咳博德特氏菌Tohama和18323的图谱一致，这使人们能够得出克隆序列具有明确保守性的结论。所获得的初步数据使得绘制与腺苷酸环化酶、凝集素2和百日咳毒素基因相邻的RSBP1同源序列成为可能。文中提出了RSBP1元件在百日咳博德特氏菌毒力调节中的可能作用。
