p73 G4C14 到 A4T14 多态性与结直肠癌风险和生存相关。
p73 G4C14 to A4T14 polymorphism is associated with colorectal cancer risk and survival.
机构信息
Department of Clinical Laboratory Science College of Health Sciences, Catholic University of Pusan, Busan 609-757, South Korea.
出版信息
World J Gastroenterol. 2010 Sep 21;16(35):4448-54. doi: 10.3748/wjg.v16.i35.4448.
AIM
To analyze the association between the p73 G4C14-to-A4T14 polymorphism (a.k.a., the GC/AT variation) and colorectal cancer risk and survival in the Korean population, and to evaluate the relationships between p73 polymorphism and the p73 protein expression or clinicopathological characteristics of colorectal cancer.
METHODS
Three hundred and eighty-three histologically confirmed cases and 469 healthy controls, recruited at one teaching hospital in Pusan, Korea from 2001 and 2007, were genotyped for p73 G4C14-to-A4T14 by PCR with confronting two-pair primers (PCR-CTPP) and the expression profile of p73 in cancer tissues (n = 383) was analyzed by immunohistochemistry.
RESULTS
Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression model adjusted for age and gender. Compared with the GC/GC genotypes, the GC/AT and AT/AT genotypes were significantly associated with colorectal cancer risk (GC/AT vs GC/GC: OR = 1.46, 95% CI: 1.10-1.94; AT/AT vs GC/GC: 1.72, 0.98-3.03; P(trend) = 0.01). When stratified by age and gender, the association was restricted to those less than 60 years of age (GC/AT or AT/AT vs GC/GC: 2.22, 1.39-3.55) and male (GC/AT or AT/AT vs GC/GC: 1.91, 1.31-2.77). The expression of p73 was associated with invasion depth (P = 0.003) and advanced Duke's stage (P = 0.06) of colorectal cancer. The patients with the GC/GC genotype were associated with worse survival compared with those with the other genotypes (P = 0.02). However, no significant relationship was observed between the p73 G4C14-to-A4T14 polymorphism and p73 protein expression in cancer tissues.
CONCLUSION
Our results suggest that the p73 GC/AT polymorphism is associated with an increased colorectal cancer risk and survival in the Korean population.
目的
分析 p73 G4C14 到 A4T14 多态性（又称 GC/AT 变异）与韩国人群结直肠癌风险和生存的关联，并评估 p73 多态性与 p73 蛋白表达或结直肠癌临床病理特征的关系。
方法
2001 年至 2007 年，在韩国釜山的一家教学医院，共招募了 383 例经组织学证实的病例和 469 例健康对照者，通过聚合酶链反应与两对引物（PCR-CTPP）对 p73 G4C14 到 A4T14 进行基因分型，并通过免疫组织化学分析癌症组织中 p73 的表达谱（n=383）。
结果
通过调整年龄和性别后的非条件逻辑回归模型计算比值比（ORs）和 95%置信区间（CIs）。与 GC/GC 基因型相比，GC/AT 和 AT/AT 基因型与结直肠癌风险显著相关（GC/AT 与 GC/GC：OR=1.46，95%CI：1.10-1.94；AT/AT 与 GC/GC：1.72，0.98-3.03；P（趋势）=0.01）。按年龄和性别分层后，该关联仅限于年龄小于 60 岁（GC/AT 或 AT/AT 与 GC/GC：2.22，1.39-3.55）和男性（GC/AT 或 AT/AT 与 GC/GC：1.91，1.31-2.77）。p73 的表达与结直肠癌的浸润深度（P=0.003）和进展期 Duke 分期（P=0.06）相关。GC/GC 基因型的患者与其他基因型的患者相比，生存情况较差（P=0.02）。然而，在癌症组织中，p73 G4C14 到 A4T14 多态性与 p73 蛋白表达之间没有观察到显著的关系。
结论
我们的结果表明，p73 GC/AT 多态性与韩国人群结直肠癌风险和生存增加有关。
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