Mwangi D M, Hopkins J, Luckins A G
Centre for Tropical Veterinary Medicine, University of Edinburgh.
Parasite Immunol. 1990 Nov;12(6):647-58. doi: 10.1111/j.1365-3024.1990.tb00994.x.
Mononuclear cell subpopulations in local skin reactions (chancres) in sheep infected with metacyclic forms of Trypanosoma congolense were studied by indirect immunoperoxidase staining using a panel of monoclonal antibodies (MoAbs) specific for ovine leucocyte subsets. Morphometric analysis revealed significant increases in numbers of cells expressing CD5, CD4, CD8, CD45R (mainly B cells), major histocompatibility complex (MHC) class II antigens, Fc receptors (FcR) on macrophages (VPM32) and FcR on B cells and macrophages (VPM33) from five days post-infection. B cells which also expressed MHC class II were found mainly in dense aggregates. The CD4/CD8 ratios were raised over pre-infection levels at 5-7 days post-infection. In sheep which had been infected, treated with trypanocidal drugs and then challenged with an heterologous serodeme of T. congolense, changes in cellular phenotype kinetics were similar to those seen in the skin in primary infections. Sheep superinfected with either an homologous or an heterologous, T. congolense serodeme showed only mild cellular infiltration and slight increases in various cellular phenotypes at the sites of inoculation.
利用一组针对绵羊白细胞亚群的单克隆抗体（MoAbs），通过间接免疫过氧化物酶染色法研究了感染刚果锥虫循环后期形态的绵羊局部皮肤反应（下疳）中的单核细胞亚群。形态计量分析显示，感染后5天起，表达CD5、CD4、CD8、CD45R（主要为B细胞）、主要组织相容性复合体（MHC）II类抗原、巨噬细胞上的Fc受体（FcR，VPM32）以及B细胞和巨噬细胞上的FcR（VPM33）的细胞数量显著增加。同时表达MHC II类抗原的B细胞主要见于致密聚集物中。感染后5 - 7天，CD4/CD8比值高于感染前水平。在已感染、接受杀锥虫药物治疗然后用刚果锥虫异源血清型攻击的绵羊中，细胞表型动力学变化与初次感染时皮肤中的变化相似。用同源或异源刚果锥虫血清型再次感染的绵羊在接种部位仅表现出轻微的细胞浸润和各种细胞表型的轻微增加。
