黏蛋白基因表达与小鼠中耳上皮
Mucin gene expression and mouse middle ear epithelium.
作者信息
Kerschner Joseph E, Li Jizhen, Tsushiya Katsuyuki, Khampang P
机构信息
Division of Pediatric Otolaryngology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W Wisconsin Avenue, Milwaukee, WI 53226, United States.
出版信息
Int J Pediatr Otorhinolaryngol. 2010 Aug;74(8):864-8. doi: 10.1016/j.ijporl.2010.04.014.
OBJECTIVES
To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).
METHODS
MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.
RESULTS
Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.
CONCLUSION
Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.
目的
在培养的小鼠中耳上皮细胞（MMEEC）体外模型中研究最近鉴定出的人类黏蛋白基因的表达情况。
方法
建立MMEEC，提取RNA并设计引物用于逆转录聚合酶链反应（RT-PCR），以评估黏蛋白基因Muc1、Muc2、Muc3、Muc4、Muc5AC、Muc5B、Muc6、Muc7、Muc8、Muc9、Muc10、Muc11/12、Muc13、Muc15、Muc16、Muc17、Muc18、Muc19和Muc20的表达。
结果
在MMEEC中鉴定并表达了黏蛋白基因Muc1、Muc2、Muc3、Muc4、Muc5AC、Muc5B、Muc9、Muc10、Muc13、Muc15、Muc16、Muc18、Muc19和Muc20。未鉴定出基因Muc6、Muc7、Muc8、Muc11/12和Muc1。
结论
最近在人类中耳上皮（MEE）和毛丝鼠MEE中鉴定出的许多黏蛋白基因也在MMEEC中表达。然而，表达存在差异，这可能对利用各种动物模型研究中耳生理学和发病机制有影响；特别是与黏蛋白基因表达相关的方面。
Zotero 插件