分泌性中耳炎积液中黏蛋白基因的表达
Mucin gene expression in the effusions of otitis media with effusion.
作者信息
Takeuchi Kazuhiko, Yagawa Mayuko, Ishinaga Hajime, Kishioka Chikako, Harada Teruhiko, Majima Yuichi
机构信息
Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan.
出版信息
Int J Pediatr Otorhinolaryngol. 2003 Jan;67(1):53-8. doi: 10.1016/s0165-5876(02)00361-0.
OBJECTIVES
The purpose of the study is to know if mucin gene expression can be detected in the middle ear effusion and if so, which mucin genes are expressed in the effusions.
METHODS
Mucin gene expression in the middle ear effusions obtained from five patients with otitis media with effusion were analyzed by reverse transcription-polymerase chain reaction. Ribonucleic acids (RNAs) were extracted from the effusion and the expression of 12 mucin genes was analyzed by reverse transcription-polymerase chain reaction.
RESULTS
Mucin gene expression examined by reverse transcription-polymerase chain reaction indicated the expression of MUC1, MUC4, MUC5AC, MUC6, MUC7, MUC8, MUC9, MUC11 and MUC12 mRNA in the effusion. This mucin gene expression was similar to that in BEAS-2B cell, a bronchial epithelial cell line.
CONCLUSION
Middle ear effusion can give us valuable information on mucin gene expression in the middle ear. There is similarity between mucin gene expression in the middle ear effusion and that in the bronchial epithelia.
目的
本研究的目的是了解中耳积液中是否能检测到黏蛋白基因表达，若能检测到，积液中表达的是哪些黏蛋白基因。
方法
采用逆转录-聚合酶链反应分析5例分泌性中耳炎患者中耳积液中的黏蛋白基因表达。从积液中提取核糖核酸（RNA），并通过逆转录-聚合酶链反应分析12种黏蛋白基因的表达。
结果
逆转录-聚合酶链反应检测的黏蛋白基因表达表明，积液中存在MUC1、MUC4、MUC5AC、MUC6、MUC7、MUC8、MUC9、MUC11和MUC12 mRNA的表达。这种黏蛋白基因表达与支气管上皮细胞系BEAS-2B细胞中的表达相似。
结论
中耳积液可为我们提供中耳黏蛋白基因表达的有价值信息。中耳积液中的黏蛋白基因表达与支气管上皮中的表达存在相似性。
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