Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Cornea. 2010 Dec;29(12):1436-9. doi: 10.1097/ICO.0b013e3181d3d69d.
We detected herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), varicella zoster virus (VZV), and cytomegalovirus (CMV) DNAs in recipient corneal buttons taken at the time of penetrating keratoplasty.
Twenty-seven corneal buttons were obtained from 27 patients (10 men and 17 women), 7 of whom had a history of HSV keratitis. Excised corneal buttons were immediately frozen in liquid nitrogen in the operating theater and then stored at -80°C until DNA extraction. The detection of HSV-1, HSV-2, VZV, and CMV DNAs was carried out by a nested polymerase chain reaction (PCR) method. The genome copy numbers for the nested PCR-positive samples were subsequently quantified by real-time PCR.
HSV-1, HSV-2, VZV, and CMV DNAs were detected in 10, 1, 9, and 2 of the 27 recipient corneal buttons, respectively. HSV-1 or HSV-2 DNAs were also detected in 5 of 7 patients with a history of HSV keratitis. Both CMV-positive patients (patients 2 and 3) had ocular pemphigoid. Among the nested PCR-positive samples, 2 HSV-1, 1 HSV-2, 1 VZV, and 1 CMV sample could be quantified by real-time PCR. Copy numbers ranged from 19 to 928 copies.
All 4 herpesviruses, including CMV, were detected in the corneal buttons. The relationship between CMV in the cornea and ocular diseases of the anterior segment should be further evaluated.
我们在穿透性角膜移植术中，检测了受体角膜纽扣中单纯疱疹病毒 1 型（HSV-1）和 2 型（HSV-2）、水痘带状疱疹病毒（VZV）和巨细胞病毒（CMV）的 DNA。
从 27 例患者（10 名男性和 17 名女性）的 27 个角膜纽扣中获得样本，其中 7 例有单纯疱疹性角膜炎病史。在手术室中，立即将切除的角膜纽扣用液氮冷冻，然后储存在-80°C 直到提取 DNA。通过巢式聚合酶链反应（PCR）方法检测 HSV-1、HSV-2、VZV 和 CMV DNA。随后通过实时 PCR 定量分析巢式 PCR 阳性样本的基因组拷贝数。
在 27 个受体角膜纽扣中，分别检测到 HSV-1、HSV-2、VZV 和 CMV DNA，10、1、9 和 2 个。7 例有单纯疱疹性角膜炎病史的患者中，也检测到 HSV-1 或 HSV-2 DNA。2 例 CMV 阳性患者（患者 2 和 3）均患有眼疱疹性类天疱疮。在巢式 PCR 阳性样本中，通过实时 PCR 可定量检测到 2 个 HSV-1、1 个 HSV-2、1 个 VZV 和 1 个 CMV 样本。拷贝数范围从 19 到 928 拷贝。
在角膜纽扣中检测到所有 4 种疱疹病毒，包括 CMV。应进一步评估角膜中 CMV 与眼前段眼部疾病的关系。
