Cyr61（CCN1）在人口腔鳞状细胞癌中的表达：预后不良的独立标志物。

Expression of Cyr61 (CCN1) in human oral squamous cell carcinoma: An independent marker for poor prognosis.

机构信息

Department of Dentistry, School of Dentistry, College of Medicine, National Taiwan University Medical Center, Taipei, Taiwan.

出版信息

Head Neck. 2010 Dec;32(12):1665-73. doi: 10.1002/hed.21381.

DOI:10.1002/hed.21381

PMID:20848406

Abstract

BACKGROUND

Cysteine-rich 61 (Cyr61 [CCN1]) has disparate functions in tumorigenesis that are dependent on the cell types. The aim of the study was to investigate its role in the growth of oral squamous cell carcinoma (SCC).

METHODS

The study used immunohistochemistry to examine Cyr61 expression in 93 oral SCC specimens and assessed the effect of Cyr61 overexpression on proliferation and migration of oral SCC cells in vitro and xenograft growth in severe combined immunodeficient (SCID) mice.

RESULTS

High expression of Cyr61 significantly correlated with large tumor size (p = .009) and advanced tumor stage (p = .036). Multivariate analysis revealed that high Cyr61 (relative risk [RR] 2.44, 95% confidence interval [CI] 1.209-4.95, p = .010) significantly correlated with mortality. Forced expression of Cyr61 stimulated the motility and growth of Ca9-22 cells in vitro and enhanced xenograft growth in SCID mice.

CONCLUSIONS

Cyr61 is a positive growth modulator of oral SCC and Cyr61 overexpression is an independent prognostic indicator for patients with oral SCC.

摘要

背景

富含半胱氨酸的 61 型（Cysteine-rich 61，Cyr61 [CCN1]）在肿瘤发生中具有不同的功能，其功能取决于细胞类型。本研究旨在探讨其在口腔鳞状细胞癌（Oral squamous cell carcinoma，SCC）生长中的作用。

方法

本研究采用免疫组织化学方法检测 93 例口腔 SCC 标本中 Cyr61 的表达，并评估 Cyr61 过表达对体外口腔 SCC 细胞增殖和迁移以及严重联合免疫缺陷（severe combined immunodeficient，SCID）小鼠异种移植生长的影响。

结果

Cyr61 高表达与肿瘤体积大（p =.009）和肿瘤分期晚显著相关（p =.036）。多因素分析显示，Cyr61 高表达（相对风险 [relative risk，RR] 2.44，95%置信区间 [confidence interval，CI] 1.209-4.95，p =.010）与死亡率显著相关。Cyr61 的强制表达可刺激 Ca9-22 细胞在体外的运动和生长，并增强 SCID 小鼠的异种移植生长。