CD44+ 癌症干细胞样细胞在头颈部鳞状细胞癌中的免疫调节特性。
Immunoregulatory properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck.
机构信息
Department of Otolaryngology-Head and Neck Surgery, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, 409-3898, Japan.
出版信息
Head Neck. 2011 Feb;33(2):208-15. doi: 10.1002/hed.21420.
BACKGROUND
CD44 was found as a surface marker in cancer stem cell (CSC) of squamous cell carcinoma of the head and neck (SCCHN); however, the immunologic properties of such CSCs have not yet been elucidated.
METHODS
The immunologic properties of CD44+ cancer stem-like cells were compared with those of CD44- cells using flow cytometry and enzyme-linked immunosorbent assay.
RESULTS
CD44+ cells exhibited weak HLA-A2 and class II expression. Interestingly, downregulation of transporter antigen processing (TAP)2 was found in CD44+ cells. The CD44+ cell population produced significantly higher levels of interleukin (IL)-8, granulocyte colony-stimulating factor (G-CSF), and transforming growth factor (TGF)-β than the CD44- cell population. Moreover, CD44+ cells have been shown to not only more strongly inhibit T-cell proliferation, but also to more efficiently inhibit regulatory T cells (Treg cells) and myeloid-derived suppressor cells (MDSC) as compared with CD44- cells. Additionally, CD44+ cells suppressed Th1 responses and enhanced regulatory T cell responses.
CONCLUSION
CSCs might have higher malignant potential with numerous escape strategies from immune attack.
背景
CD44 作为头颈部鳞状细胞癌（SCCHN）癌症干细胞（CSC）的表面标志物被发现；然而，这些 CSCs 的免疫特性尚未阐明。
方法
使用流式细胞术和酶联免疫吸附试验比较了 CD44+癌症类干细胞与 CD44-细胞的免疫特性。
结果
CD44+细胞表现出较弱的 HLA-A2 和 II 类表达。有趣的是，在 CD44+细胞中发现了转运体抗原加工（TAP）2 的下调。与 CD44-细胞群相比，CD44+细胞群产生的白细胞介素（IL）-8、粒细胞集落刺激因子（G-CSF）和转化生长因子（TGF）-β水平明显更高。此外，与 CD44-细胞相比，CD44+细胞不仅更强烈地抑制 T 细胞增殖，而且更有效地抑制调节性 T 细胞（Treg 细胞）和髓样来源的抑制细胞（MDSC）。此外，CD44+细胞抑制 Th1 反应并增强调节性 T 细胞反应。
结论
CSC 可能具有更高的恶性潜能，并具有许多逃避免疫攻击的策略。
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