慢性槟榔碱处理的口腔癌细胞中 Ches1 和其他新基因的下调。
Downregulation of Ches1 and other novel genes in oral cancer cells chronically exposed to areca nut extract.
机构信息
Graduate School of Medical Biotechnology, Chang Gung University, Taoyuan 333, Taiwan, Providence of China.
出版信息
Head Neck. 2011 Feb;33(2):257-66. doi: 10.1002/hed.21442.
BACKGROUND
This study was undertaken to identify the genes in response to areca nut extract, a potential carcinogen of oral cancer.
METHODS
Two oral cancer sublines chronically treated with areca nut extract were established. Methods such as microarray and immunohistochemistry were used to screen and validate the genes' altered expressions in areca nut extract-sublines or in cancer tissues.
RESULTS
A total of 35 genes were differentially expressed in both sublines. Several functional pathways were significantly altered. Six genes were confirmed over 2-fold of changes, including Ches1. Functional analyses showed that overexpression of Ches1 suppressed cell growth and arrested cells in the G2/M phase. Consistently, this gene has reduced expression in 52% of oral cancer tissues, which was significantly correlated with the areca nut chewing habit of patients (p = .04).
CONCLUSION
We identified 35 candidates and validated 6 genes that may be associated with areca nut-induced oral cancer. Loss of Ches1 may be attributed to areca nut extract-induced oral carcinogenesis.
背景
本研究旨在鉴定槟榔提取物（口腔癌的潜在致癌物质）作用下的基因。
方法
建立了两种经槟榔提取物慢性处理的口腔癌细胞亚系。采用微阵列和免疫组织化学等方法筛选和验证槟榔提取物亚系或癌症组织中基因表达的改变。
结果
两个亚系中共有 35 个基因表达差异。多个功能途径发生明显改变。有 6 个基因的变化超过 2 倍，包括 Ches1。功能分析显示 Ches1 的过表达抑制细胞生长并将细胞阻滞在 G2/M 期。一致的是，该基因在 52%的口腔癌组织中表达降低，与患者咀嚼槟榔的习惯显著相关（p=0.04）。
结论
我们鉴定了 35 个候选基因，并验证了 6 个可能与槟榔诱导的口腔癌相关的基因。Ches1 的缺失可能归因于槟榔提取物诱导的口腔癌发生。
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