Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Chembiochem. 2010 Oct 18;11(15):2089-91. doi: 10.1002/cbic.201000234.
Recently we reported that the linear tetracysteine sequence preferred by FlAsH and ReAsH could be split between two members of a protein partnership or two approximated regions of a folded protein while maintaining high affinity and brightness. Here we show that this tool–bipartite tetracysteine display–facilitates the design of E2, an encodable, site-selective, Src-family kinase sensor.
最近我们报道了，FlAsH 和 ReAsH 偏爱的线性四半胱氨酸序列可以在蛋白质伴侣的两个成员之间或折叠蛋白质的两个近似区域之间分裂，同时保持高亲和力和亮度。在这里，我们展示了这种工具——双部分四半胱氨酸展示——有助于 E2 的设计，E2 是一种可编码、位点选择性、Src 家族激酶传感器。
