Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Curr Opin Chem Biol. 2009 Oct;13(4):392-7. doi: 10.1016/j.cbpa.2009.07.009. Epub 2009 Aug 12.
Dynamic post-translational modifications (PTMs) regulate and diversify protein properties and cellular behaviors. Real-time monitoring of these modifications has been made possible with biosensors based on fluorescent proteins (FPs) and fluorescence resonance energy transfer (FRET), which can provide spatiotemporal information of PTMs with little perturbation to the cellular environment. In this review, we highlight available fluorescent biosensors applicable to detect PTMs in living cells and how they have shed light on biological questions that have been difficult to address otherwise. In addition, we also provide discussions about various engineering strategies for overcoming potential challenges associated with the development and application of such biosensors.
动态翻译后修饰（PTMs）调节和多样化蛋白质的性质和细胞行为。基于荧光蛋白（FPs）和荧光共振能量转移（FRET）的生物传感器使这些修饰的实时监测成为可能，它们可以在不对细胞环境造成干扰的情况下提供 PTMs 的时空信息。在这篇综述中，我们重点介绍了可用于检测活细胞中 PTM 的荧光生物传感器，并展示了它们如何解决其他方法难以解决的生物学问题。此外，我们还讨论了各种工程策略，以克服与这类生物传感器的开发和应用相关的潜在挑战。
