使用元件标记底物的多重蛋白酶分析。
Multiplexed protease assays using element-tagged substrates.
机构信息
Department of Chemistry, University of Toronto, Ontario, Canada.
出版信息
Anal Biochem. 2011 Jan 1;408(1):157-9. doi: 10.1016/j.ab.2010.09.008. Epub 2010 Sep 16.
Abstract
Inductively coupled plasma-mass spectrometry (ICP-MS)-based assays lend themselves to multiplexing due to the high resolution between mass channels, the sensitivity, and the reliability of the technique. Here the potential of ICP-MS-based protease assays is demonstrated with a quadruplex assay of cysteine proteases and metalloproteases. Four orthogonal peptide substrates were synthesized for the proteases calpain-1, caspase-3, matrix metalloprotease-9 (MMP-9), and a disintegrin and metalloprotease-10 (ADAM10). Each substrate carries a biotin tag at the C terminus and a diethylenetriaminepentaacetic acid (DTPA)-based lanthanide complex at the N terminus. The results demonstrate that this is a simple and reproducible analysis technique with excellent correlation between the single and multiplex assay formats.
摘要
基于电感耦合等离子体质谱法（ICP-MS）的测定方法由于质量通道之间的高分辨率、技术的灵敏度和可靠性而适合多重检测。本文展示了基于 ICP-MS 的蛋白酶测定方法的潜力，采用四重检测方法对半胱氨酸蛋白酶和金属蛋白酶进行检测。针对钙蛋白酶-1、半胱天冬酶-3、基质金属蛋白酶-9（MMP-9）和金属蛋白酶 10（ADAM10）这 4 种蛋白酶，合成了 4 种正交肽底物。每个底物的 C 端带有生物素标记，N 端带有二亚乙基三胺五乙酸（DTPA）基镧系元素络合物。结果表明，这是一种简单且可重现的分析技术，单重和多重测定方法之间具有极好的相关性。
相似文献
1
Multiplexed protease assays using element-tagged substrates.使用元件标记底物的多重蛋白酶分析。
Anal Biochem. 2011 Jan 1;408(1):157-9. doi: 10.1016/j.ab.2010.09.008. Epub 2010 Sep 16.
2
Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8.鉴定金属蛋白酶-解整合素ADAM8介导的胞外域脱落的候选底物。
Biol Chem. 2006 Mar;387(3):337-46. doi: 10.1515/BC.2006.045.
3
Development of inductively coupled plasma-mass spectrometry-based protease assays.基于电感耦合等离子体质谱法的蛋白酶检测方法的建立。
Anal Biochem. 2010 Mar 1;398(1):93-8. doi: 10.1016/j.ab.2009.11.010. Epub 2009 Nov 11.
4
Discovery and characterization of a novel, widely expressed metalloprotease, ADAMTS10, and its proteolytic activation.一种新型、广泛表达的金属蛋白酶ADAMTS10的发现与鉴定及其蛋白水解激活
J Biol Chem. 2004 Dec 3;279(49):51208-17. doi: 10.1074/jbc.M409036200. Epub 2004 Sep 7.
5
Snake venom metalloproteases--structure and function of catalytic and disintegrin domains.蛇毒金属蛋白酶——催化结构域和去整合素结构域的结构与功能
Comp Biochem Physiol C Toxicol Pharmacol. 2006 Mar-Apr;142(3-4):328-346. doi: 10.1016/j.cbpc.2005.11.005. Epub 2006 Jan 24.
6
Transcriptomic mapping of the metzincin landscape in human trophoblasts.人类滋养层细胞中金属蛋白酶景观的转录组图谱
Gene Expr Patterns. 2022 Dec;46:119283. doi: 10.1016/j.gep.2022.119283. Epub 2022 Oct 25.
7
Molecular cloning and sequence analysis of ussurin, a new metalloproteinases/disintegrin from Gloydius ussuriensis.乌苏里蝮蛇新金属蛋白酶/解整合素——乌苏里素的分子克隆与序列分析
Sheng Wu Gong Cheng Xue Bao. 2003 May;19(3):353-7.
8
A novel metalloprotease from Gloydius halys venom induces endothelial cell apoptosis through its protease and disintegrin-like domains.一种来自东亚钳蝎毒液的新型金属蛋白酶通过其蛋白酶和类解整合素结构域诱导内皮细胞凋亡。
J Biochem. 2003 Nov;134(5):739-49. doi: 10.1093/jb/mvg202.
9
Discovery of novel inhibitors of a disintegrin and metalloprotease 17 (ADAM17) using glycosylated and non-glycosylated substrates.使用糖基化和非糖基化底物发现新型解整合素金属蛋白酶 17（ADAM17）抑制剂。
J Biol Chem. 2012 Oct 19;287(43):36473-87. doi: 10.1074/jbc.M112.389114. Epub 2012 Aug 27.
10
Expression and enzymatic activity of human disintegrin and metalloproteinase ADAM19/meltrin beta.人去整合素金属蛋白酶ADAM19/β-解整合素金属蛋白酶的表达及酶活性
Biochem Biophys Res Commun. 2001 Jan 26;280(3):744-55. doi: 10.1006/bbrc.2000.4200.
引用本文的文献
1
Multiplexed Probing of Proteolytic Enzymes Using Mass Cytometry-Compatible Activity-Based Probes.使用质谱流式细胞仪兼容的基于活性的探针对蛋白酶进行多重探测。
J Am Chem Soc. 2020 Sep 30;142(39):16704-16715. doi: 10.1021/jacs.0c06762. Epub 2020 Sep 15.
2
In vitro methods for testing antiviral drugs.抗病毒药物的体外检测方法。
Biotechnol Adv. 2018 May-Jun;36(3):557-576. doi: 10.1016/j.biotechadv.2017.12.016. Epub 2017 Dec 29.
3
Small Molecule Active Site Directed Tools for Studying Human Caspases.用于研究人类半胱天冬酶的小分子活性位点导向工具
Chem Rev. 2015 Nov 25;115(22):12546-629. doi: 10.1021/acs.chemrev.5b00434. Epub 2015 Nov 9.
4
Elemental labelling combined with liquid chromatography inductively coupled plasma mass spectrometry for quantification of biomolecules: a review.元素标记与液相色谱-电感耦合等离子体质谱联用在生物分子定量分析中的应用：综述。
Anal Chim Acta. 2012 Oct 31;750(100):98-110. doi: 10.1016/j.aca.2012.06.040. Epub 2012 Jul 8.
5
Detection of protease activities by flash chronopotentiometry using a reversible polycation-sensitive polymeric membrane electrode.利用可逆聚阳离子敏感聚合物膜电极的闪光恒电流计时法检测蛋白酶活性。
Anal Biochem. 2011 Sep 1;416(1):67-73. doi: 10.1016/j.ab.2011.04.036. Epub 2011 Apr 29.
本文引用的文献
1
Development of inductively coupled plasma-mass spectrometry-based protease assays.基于电感耦合等离子体质谱法的蛋白酶检测方法的建立。
Anal Biochem. 2010 Mar 1;398(1):93-8. doi: 10.1016/j.ab.2009.11.010. Epub 2009 Nov 11.
2
Multiplexed determination of protein biomarkers using metal-tagged antibodies and size exclusion chromatography--inductively coupled plasma mass spectrometry.使用金属标记抗体和尺寸排阻色谱-电感耦合等离子体质谱法对蛋白质生物标志物进行多重测定。
Anal Chem. 2009 Nov 15;81(22):9440-8. doi: 10.1021/ac901853g.
3
Emerging roles of mitochondrial proteases in neurodegeneration.线粒体蛋白酶在神经退行性变中的新作用。
Biochim Biophys Acta. 2010 Jan;1797(1):1-10. doi: 10.1016/j.bbabio.2009.07.013. Epub 2009 Aug 5.
4
Matrix metalloproteinase proteomics: substrates, targets, and therapy.基质金属蛋白酶蛋白质组学：底物、靶点与治疗
Curr Opin Cell Biol. 2009 Oct;21(5):645-53. doi: 10.1016/j.ceb.2009.06.006. Epub 2009 Jul 16.
5
Short transient signals, a challenge for inductively coupled plasma mass spectrometry, a review.短瞬态信号：电感耦合等离子体质谱面临的挑战，综述
Anal Chim Acta. 2009 Feb 2;633(1):19-28. doi: 10.1016/j.aca.2008.11.041. Epub 2008 Nov 25.
6
ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.基于电感耦合等离子体质谱的糖蛋白多重分析：使用与镧系元素螯合聚合物偶联的凝集素
J Proteome Res. 2009 Feb;8(2):443-9. doi: 10.1021/pr800645r.
7
Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugation.通过内含肽介导的特异性生物共轭制备量子点纳米传感器对蛋白酶活性进行多重检测。
Anal Chem. 2008 Nov 15;80(22):8649-55. doi: 10.1021/ac801562f. Epub 2008 Oct 16.
8
Regulation of the proteolytic disintegrin metalloproteinases, the 'Sheddases'.蛋白水解性解整合素金属蛋白酶（“脱落酶”）的调控
Semin Cell Dev Biol. 2009 Apr;20(2):138-45. doi: 10.1016/j.semcdb.2008.09.004. Epub 2008 Sep 18.
9
Energy transfer-based multiplexed assay of proteases by using gold nanoparticle and quantum dot conjugates on a surface.利用表面的金纳米颗粒和量子点共轭物基于能量转移的蛋白酶多重分析
Anal Chem. 2008 Jun 15;80(12):4634-41. doi: 10.1021/ac702416e.
10
Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip.使用毛细管组装微芯片对癌细胞裂解物中的各种蛋白酶进行单滴分析。
Anal Bioanal Chem. 2008 Aug;391(7):2507-12. doi: 10.1007/s00216-008-2105-x. Epub 2008 Apr 23.
Zotero 插件