Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Viale G. P. Usberti 17/A, I-43100 Parma, Italy.
Anal Chem. 2009 Nov 15;81(22):9440-8. doi: 10.1021/ac901853g.
A liquid-phase immunoassay was developed for the simultaneous determination of five cancer biomarker proteins: alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG), carcinoembryonic antigen (CEA), ovarian tumor antigen (CA125/MUC16), and gastrointestinal tumor antigen (CA19-9). The method was based on the incubation of a serum (or tissue cytosol) with five antibodies, each labeled with a different lanthanide (Pr(3+), Eu(3+), Gd(3+), Ho(3+), and Tb(3+), respectively) followed by the specific determination of the immunocomplex formed by size exclusion chromatography with inductively coupled plasma mass spectrometric detection (SEC-ICPMS). The sensitivity of the method was comparable with that attainable by enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay with the advantages of multiplexed analysis capacity, virtually no sample preparation, and sample amount consumption, ca. 3 times lower than an ELISA test. The method was validated for the analysis of the proteins in human serum and proved to be able to discriminate ovary and uterus tumor tissue samples from those of healthy subjects.
建立了一种液相免疫分析方法，用于同时测定五种癌症生物标志物蛋白：甲胎蛋白（AFP）、人绒毛膜促性腺激素（hCG）、癌胚抗原（CEA）、卵巢肿瘤抗原（CA125/MUC16）和胃肠道肿瘤抗原（CA19-9）。该方法基于将血清（或组织胞浆）与五种抗体孵育，每种抗体分别标记一种不同的镧系元素（分别为 Pr(3+)、Eu(3+)、Gd(3+)、Ho(3+)和 Tb(3+)），然后通过尺寸排阻色谱与电感耦合等离子体质谱检测（SEC-ICPMS）特异性测定形成的免疫复合物。该方法的灵敏度与酶联免疫吸附测定（ELISA）或放射免疫测定相当，但具有多重分析能力、几乎无需样品制备以及样品消耗少的优点，约为 ELISA 检测的 1/3。该方法经过验证，可用于分析人血清中的蛋白质，并能够区分卵巢和子宫肿瘤组织样本与健康受试者的样本。
