Blanco María Teresa, Sacristán Beatriz, Lucio Leopoldo, Blanco Javier, Pérez-Giraldo Ciro, Gómez-García Antonio Cándido
Área de Microbiología, Departamento de Ciencias Biomédicas, Facultad de Medicina, Universidad de Extremadura, Badajoz, España.
Rev Iberoam Micol. 2010 Oct-Dec;27(4):195-9. doi: 10.1016/j.riam.2010.09.001. Epub 2010 Sep 16.
To evaluate virulence factors involved in the adhesion process, such as cell surface hydrophobicity (CSH), adherence to plastic capacity, adherence capacity to buccal epithelial cells (BEC), and biofilm formation, in 17 strains of C. albicans isolated from bronchial aspirates of critically ill patients.
The CSH of the strains of C. albicans was determined using the MATH method, a microbial adhesion to hydrocarbons test. The study of adherence to plastic was performed in microtitre plates in accordance with Christensen's technique. Biofilm formation was studied in polystyrene microtitre plates, according to the method of Ramage. Adherence to BEC was evaluated by quantifying the percentage of adhered yeasts to cells.
All the strains studied showed factors directly involved in adhesion, with variability in the degree of expression among them. Medium-high levels of CSH were found in 52.9% of the strains. The percentage of strains with high values in adherence to plastic was 35.3%. The most hydrophobic strains were the most adherent to plastic, with a correlation coefficient of 0.76. Of the 12 biofilm-producing strains, 6 were high producers. These strains had also high levels of CSH and adherence to plastic, with significant results. All the strains studied adhered to BEC, with results ranging widely from 45 to 157 yeasts/100 BEC, with no significant correlation with the rest of the parameters studied, although CSH was seen to be an indispensable prior requisite for adherence to cells.
CSH is a variable characteristic in C. albicans and is directly related to adherence to plastic and biofilm formation. Ease in evaluating CSH permits its quantification, and could be used as an indicator of the presence of other determinants of pathogenicity.
评估从重症患者支气管吸出物中分离出的17株白色念珠菌在黏附过程中涉及的毒力因子，如细胞表面疏水性（CSH）、对塑料的黏附能力、对颊黏膜上皮细胞（BEC）的黏附能力以及生物膜形成。
采用MATH法（一种微生物对碳氢化合物的黏附试验）测定白色念珠菌菌株的CSH。按照克里斯滕森技术在微量滴定板中进行对塑料黏附的研究。根据拉梅奇的方法，在聚苯乙烯微量滴定板中研究生物膜形成。通过量化黏附到细胞上的酵母百分比来评估对BEC的黏附。
所有研究的菌株均显示出直接参与黏附的因子，它们之间的表达程度存在差异。52.9%的菌株CSH水平为中高。对塑料黏附值高的菌株百分比为35.3%。疏水性最强的菌株对塑料的黏附性最强，相关系数为0.76。在12株产生生物膜的菌株中，6株为高产菌株。这些菌株的CSH水平和对塑料的黏附性也很高，结果具有显著性。所有研究的菌株均黏附于BEC，结果范围广泛，从每100个BEC中有45至157个酵母，与其他研究参数无显著相关性，尽管CSH被认为是黏附到细胞上必不可少的先决条件。
CSH是白色念珠菌的一个可变特征，与对塑料的黏附及生物膜形成直接相关。评估CSH简便易行，可对其进行量化，并可作为其他致病性决定因素存在的指标。
