The College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
The College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
Poult Sci. 2021 Apr;100(4):101003. doi: 10.1016/j.psj.2021.01.026. Epub 2021 Jan 16.
Previously, a fungus was isolated from a diseased pigeon group clinically suspected of being infected with Candida. The fungus was subsequently identified as Candida glabrata using morphology, physiology, biochemistry, and molecular biology testing methods. In the present study, to determine the controlling effects of Chinese herbal medicine for C. glabrata, the bacteriostatic effects of the ethanol extracts Acorus gramineus, Sophora flavescens, Polygonum hydropiper, Cassia obtusifolia, Pulsatilla chinensis, Dandelion, and Cortex phellodendri on C. glabrata in vitro were analyzed. The results showed that the minimum inhibitory concentrations (MIC) of Cortex phellodendri was 0.25 μg/μL. Meanwhile, that of S. flavescens was 32 μg/μL; C. obtusifolia was 56 μg/μL; A. gramineus and Polygonum hydropiper was 64 μg/μL; and P. chinensis was 112 μg/μL. However, MIC for Dandelion was undetectable. In addition, improved drug sensitivity tests revealed that colonies had grown after 24 h in the blank group, as well as the Polygonum hydropiper, P. chinensis, Dandelion, and ethanol groups. The colonies first appeared at the 48-hour point in the other drug-sensitive medium of Chinese herbal medicine. However, no colony growth was found in Cortex phellodendri medium, and the formation of the maximum colony diameter in that group was later than the blank group (e.g., 96 h in the blank group and 120 h in the Chinese herbal medicine group). It was observed that only 17 colony-forming units had grown in 125 μg/μL of the S. flavescens medium, which was significantly different from other groups. Also, the final colony diameter was significantly smaller than that of the other experimental groups. Therefore, it was determined that the A. gramineus, S. flavescens, Polygonum hydropiper, Cassia obtusifolia, P. chinensis, and Cortex phellodendri had certain inhibitory effects on the growth of the C. glabrata. Among those, it was observed that the Cortex phellodendri had the strongest inhibitory effects, followed by the S. flavescens. In the future, these Chinese herbal medicines are expected to be used to treat the fungal infections related to C. glabrata in poultry to improve production performance.
先前，从临床上疑似感染念珠菌的病鸽群中分离出一种真菌，随后通过形态学、生理学、生物化学和分子生物学检测方法将其鉴定为光滑念珠菌。本研究旨在探讨中草药对光滑念珠菌的控制作用，分析菖蒲、苦参、水飞蓟、决明子、白头翁、蒲公英和黄柏乙醇提取物对体外光滑念珠菌的抑菌效果。结果表明，黄柏的最小抑菌浓度（MIC）为 0.25μg/μL，苦参为 32μg/μL，决明子为 56μg/μL，菖蒲和水飞蓟均为 64μg/μL，白头翁为 112μg/μL，而蒲公英的 MIC 则无法检测。此外，药敏试验改良法显示，空白组和水飞蓟、白头翁、蒲公英及乙醇组孵育 24 h 后菌落生长，其他中草药药敏介质中，48 h 开始出现菌落，但黄柏组无菌落生长，且该组最大菌落直径形成时间晚于空白组（空白组 96 h，中草药组 120 h）。在 125μg/μL 苦参培养基中仅生长 17 个集落形成单位，与其他组差异显著，且最终菌落直径明显小于其他实验组。因此，菖蒲、苦参、水飞蓟、决明子、白头翁和黄柏对光滑念珠菌的生长有一定的抑制作用，其中黄柏抑制作用最强，苦参次之。今后有望将这些中草药用于治疗与光滑念珠菌相关的家禽真菌感染，以提高生产性能。
