Diazyme ADA 检测试剂盒在 Cobas® 6000 系统上的分析性能。
Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system.
机构信息
Hôpital d'Instruction des Armées Bégin, Service de biochimie, Toxicologie, Pharmacologie Cliniques, 69, Saint Mandé, France.
出版信息
Clin Biochem. 2010 Dec;43(18):1468-71. doi: 10.1016/j.clinbiochem.2010.09.005. Epub 2010 Sep 17.
OBJECTIVE
To evaluate the analytical performance of the Diazyme ADA assay on the Cobas® 6000 system for pleural fluid samples analysis.
DESIGN AND METHODS
Imprecision, linearity, calibration curve stability, interference, and correlation studies were completed.
RESULTS
The Diazyme ADA assay demonstrated excellent precision (CV<4%) over the analytical measurement range (0.5-117 U/L). Bilirubin above 50 μmol/L and haemoglobin above 177 μmol/L interfered with the test, inducing a negative and a positive interference respectively. The Diazyme ADA assay correlated well with the Giusti method (r(2)=0.93) but exhibited a negative bias (~ -30%).
CONCLUSIONS
The Diazyme ADA assay on the Cobas® 6000 system represents a rapid, accurate, precise and reliable method for determination of ADA activity in pleural fluid samples.
目的
评估 Diazyme ADA 检测试剂盒在 Cobas® 6000 系统上用于分析胸腔积液样本的分析性能。
设计和方法
完成了不精密度、线性、校准曲线稳定性、干扰和相关性研究。
结果
Diazyme ADA 检测试剂盒在分析测量范围内（0.5-117 U/L）表现出优异的精密度（CV<4%）。胆红素>50 μmol/L 和血红蛋白>177 μmol/L 干扰了该检测，分别导致负干扰和正干扰。Diazyme ADA 检测试剂盒与 Giusti 法相关性良好（r(2)=0.93），但表现出负偏倚（约-30%）。
结论
Cobas® 6000 系统上的 Diazyme ADA 检测试剂盒是一种快速、准确、精密和可靠的方法，用于测定胸腔积液样本中的 ADA 活性。
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