Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
Vaccine. 2010 Oct 28;28(46):7345-50. doi: 10.1016/j.vaccine.2010.09.005. Epub 2010 Sep 17.
A study to evaluate the immunogenicity and protective efficacy of a Venezuelan equine encephalitis virus (VEEV) DNA vaccine in an aerosol model of nonhuman primate infection was performed. Cynomolgus macaques vaccinated with a plasmid expressing the 26S structural genes of VEEV subtype IAB by particle-mediated epidermal delivery (PMED) developed virus-neutralizing antibodies. No serum viremia was detected in two out of three macaques vaccinated with the VEEV DNA after aerosol challenge with homologous virus, while one displayed a low viremia on a single day postchallenge. In contrast, all three macaques vaccinated with empty vector DNA developed a high viremia that persisted for at least 3 days after challenge. In addition, macaques vaccinated with the VEEV DNA had reduced febrile reactions, lymphopenia, and clinical signs of disease postchallenge as compared to negative control macaques. Therefore, although the sample size was small in this pilot study, these results indicate that a VEEV DNA vaccine administered by PMED can at least partially protect nonhuman primates against an aerosol VEEV challenge.
进行了一项研究，以评估委内瑞拉马脑炎病毒（VEEV）DNA 疫苗在非人灵长类动物感染气溶胶模型中的免疫原性和保护效力。通过粒子介导的表皮传递（PMED）用表达 VEEV 亚型 IAB 的 26S 结构基因的质粒对食蟹猴进行接种，产生了病毒中和抗体。在对同源病毒进行气溶胶攻击后，用 VEEV DNA 接种的三只猕猴中的两只未检测到血清病毒血症，而一只在攻毒后单日出现低病毒血症。相比之下，用空载体 DNA 接种的所有三只猕猴均产生了持续至少 3 天的高病毒血症。此外，与阴性对照猕猴相比，用 VEEV DNA 接种的猕猴在攻毒后发热反应、淋巴细胞减少和疾病临床体征减少。因此，尽管这项初步研究的样本量较小，但这些结果表明，通过 PMED 接种的 VEEV DNA 疫苗至少可以部分保护非人灵长类动物免受气溶胶 VEEV 挑战。
