底物、金属和模板对正磷酸盐和焦磷酸盐抑制噬菌体 - qβ核糖核酸聚合酶的影响。
Substrate, metal and template effects on inhibition of bacteriophage-qbeta ribonucleic acid polymerase by ortho- and pyro-phosphate.
作者信息
Brooks R R, Andersen J A
出版信息
Biochem J. 1978 Jun 1;171(3):725-32. doi: 10.1042/bj1710725.
Abstract
Two reactions of bacteriophage-Qbeta RNA polymerase with synthetic templates were characterized and used to study the effects of substrate, metal and template on inhibition by Pi and PPi. Analysis of the poly(C)-dependent reaction yielded results on kinetics, GTP-dependence, preference for Mn2+ over Mg2+, and Michaelis constants for template similar to those in the literature. New data are provided for the poly(U2,C)-dependent reaction. Our results suggest that GTP and Mn2+ can form relatively stable complexes with the polymerase and that such complexes change the interaction of the enzyme with the inhibitors, Pi and PPi.
摘要
对噬菌体-Qβ RNA聚合酶与合成模板的两种反应进行了表征，并用于研究底物、金属和模板对Pi和PPi抑制作用的影响。对依赖于聚（C）的反应进行分析，得出了有关动力学、GTP依赖性、对Mn2+比对Mg2+的偏好性以及模板的米氏常数等结果，这些结果与文献中的结果相似。提供了关于依赖于聚（U2，C）的反应的新数据。我们的结果表明，GTP和Mn2+可以与聚合酶形成相对稳定的复合物，并且这种复合物会改变酶与抑制剂Pi和PPi的相互作用。
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Substrate, metal and template effects on inhibition of bacteriophage-qbeta ribonucleic acid polymerase by ortho- and pyro-phosphate.底物、金属和模板对正磷酸盐和焦磷酸盐抑制噬菌体 - qβ核糖核酸聚合酶的影响。
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