Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, People's Republic of China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, People's Republic of China.
Vet Microbiol. 2020 Jan;240:108538. doi: 10.1016/j.vetmic.2019.108538. Epub 2019 Nov 29.
Human salmonellosis caused by the consumption of eggs and chicken meat contaminated with Salmonella Enteritidis has become a continuing public health concern worldwide. In this study we adopted whole genome sequencing (WGS) to determine the genetic relationship and antimicrobial resistance of S. enterica strains isolated from a poultry breeding enterprise that consists of one breeding chicken farm, one egg hatchery and one commercial chicken farm. A total of 148 S. enterica including 147 S. Enteritidis strains were isolated from 2100 fecal swab samples, with 16 (5.3 %, 16/300) from breeding chicken farm, 38 (4.2 %, 38/900) from egg hatchery and 94 (10.4 %, 94/900) from commercial chicken farm. WGS revealed that all 147 S. Enteritidis strains belonged to ST11, and further divided into 4 different ribosomal STs and 64 core genome STs. Single nucleotide polymorphism typing suggested the presence of the vertical transmission of S. Enteritidis from breeding chicken to commercial chicken. Three different antimicrobial-resistant plasmids including one bla-carrying plasmid and two virulence-resistance plasmids were characterized, resulting in the heterogeneous antimicrobial resistance of clonally related S. Enteritidis strains. Routine surveillance in breeding chicken farms is conducive to the control of S. Enteritidis from farm to fork.
人因食用受肠炎沙门氏菌污染的鸡蛋和鸡肉而感染沙门氏菌病已成为全球持续存在的公共卫生关注问题。在本研究中，我们采用全基因组测序（WGS）来确定来自一个由一个种鸡场、一个蛋鸡孵化场和一个商品鸡场组成的家禽养殖企业的分离株的遗传关系和抗药性。从 2100 份粪便拭子样本中分离出了 148 株肠炎沙门氏菌，包括 147 株肠炎沙门氏菌，其中 16 株（5.3%，16/300）来自种鸡场，38 株（4.2%，38/900）来自蛋鸡孵化场，94 株（10.4%，94/900）来自商品鸡场。WGS 显示，所有 147 株肠炎沙门氏菌均属于 ST11，进一步分为 4 种不同的核糖体 ST 和 64 种核心基因组 ST。单核苷酸多态性分型提示肠炎沙门氏菌从种鸡垂直传播到商品鸡。鉴定出了 3 种不同的耐药质粒，包括一种携带 bla 的质粒和两种毒力耐药质粒，导致克隆相关的肠炎沙门氏菌菌株具有异质性的耐药性。在种鸡场进行常规监测有助于控制从农场到餐桌的肠炎沙门氏菌。
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