Mokgophi Thelma M, Gcebe Nomakorinte, Fasina Folorunso, Adesiyun Abiodun A
Department of Production Animal Studies, University of Pretoria, Private Bag X 04, Onderstepoort, Pretoria 0110, South Africa.
Agricultural Research Council-Bacteriology and Zoonotic Diseases Diagnostic Laboratory, Onderstepoort Veterinary Research, Private Bag X 05, Onderstepoort, Pretoria 0110, South Africa.
Pathogens. 2021 Mar 1;10(3):273. doi: 10.3390/pathogens10030273.
The study determined the antimicrobial resistance profiles of on chickens processed and retailed at outlets of the informal markets in Gauteng province, South Africa. The study also investigated the relationship of antimicrobial resistant to the source and type of samples and their serotypes. Carcass swabs, cloacal swabs and carcass drips were randomly collected from each of 151 slaughtered chickens from six townships. Isolation and identification were performed using standard and polymerase chain reaction (PCR) methods. The disc diffusion method was used to determine the resistance of isolates to 16 antimicrobial agents and PCR to determine their serovars. Ninety-eight (64.9%) of the 151 chickens were contaminated with of which 94.9% (93/98) were resistant serovars. The frequency of antimicrobial resistance of isolates was high to erythromycin (94.9%) and spectinomycin (82.7%) but was low to ciprofloxacin (1.0%) and norfloxacin (1.0%) ( < 0.05). All 170 isolates of tested exhibited resistance to one or more antimicrobial agents and the frequency varied significantly ( < 0.05) across the townships, the type of samples and the serovars. The prevalence of multidrug resistance (MDR) in was 81.8% (139/170). Our findings pose zoonotic, food safety and therapeutic risks to workers and consumers of undercooked, contaminated chickens from these outlets.
该研究确定了南非豪登省非正式市场网点加工和零售的鸡肉上的抗菌药物耐药性概况。该研究还调查了抗菌药物耐药性与样本来源、类型及其血清型之间的关系。从六个城镇的151只屠宰鸡中随机采集胴体拭子、泄殖腔拭子和胴体滴液。使用标准方法和聚合酶链反应（PCR）方法进行分离和鉴定。采用纸片扩散法测定分离株对16种抗菌药物的耐药性，采用PCR法测定其血清型。151只鸡中有98只（64.9%）被[某种细菌名称未给出]污染，其中94.9%（93/98）为耐药血清型。[某种细菌名称未给出]分离株对红霉素（94.9%）和壮观霉素（82.7%）的抗菌药物耐药频率较高，但对环丙沙星（1.0%）和诺氟沙星（1.0%）的耐药频率较低（P<0.05）。所有检测的170株[某种细菌名称未给出]分离株均对一种或多种抗菌药物耐药，且不同城镇、样本类型和血清型之间的耐药频率差异显著（P<0.05）。[某种细菌名称未给出]的多重耐药（MDR）患病率为81.8%（139/170）。我们的研究结果对这些网点未煮熟、受污染鸡肉的工人和消费者构成了人畜共患病、食品安全和治疗风险。
