Hou D X, Maeda Y, Okamoto S, Hashiguchi T
Department of Animal Science, Faculty of Agriculture, Kagoshima University, Japan.
Biochem Genet. 1990 Dec;28(11-12):571-6. doi: 10.1007/BF00553949.
A chymotrypsinogen showing the phenotype AA of chymotrypsin was purified from quail pancreas, and its chromatographic behavior, molecular weight, and electrophoretic mobility were very similar to those of another chymotrypsinogen, showing the aa phenotype of chymotrypsin. However, after activation, this chymotrypsinogen from AA showed band 5 of chymotrypsin, while the other chymotrypsinogen from aa did not. Peptide mapping demonstrated that the molecular structures of the two chymotrypsinogens are different. It was recognized that chymotrypsin variants result from activated products of different molecular structures of chymotrypsinogens.
从鹌鹑胰腺中纯化出一种显示胰凝乳蛋白酶AA表型的胰凝乳蛋白酶原，其色谱行为、分子量和电泳迁移率与另一种显示胰凝乳蛋白酶aa表型的胰凝乳蛋白酶原非常相似。然而，激活后，来自AA的这种胰凝乳蛋白酶原显示出胰凝乳蛋白酶的条带5，而来自aa的另一种胰凝乳蛋白酶原则没有。肽图谱分析表明这两种胰凝乳蛋白酶原的分子结构不同。人们认识到胰凝乳蛋白酶变体是由不同分子结构的胰凝乳蛋白酶原的激活产物产生的。
