Yadav Dhirendra Singh, Devi Thoudam Regina, Ihsan Rakhshan, Mishra Ashwani Kumar, Kaushal Mishi, Chauhan Pradeep Singh, Bagadi Sarangadhara A R, Sharma Jagannath, Zamoawia Eric, Verma Yogesh, Nandkumar Ambakumar, Saxena Sunita, Kapur Sujala
Institute of Pathology, Indian Council of Medical Research (ICMR), New Delhi, India.
Genet Test Mol Biomarkers. 2010 Oct;14(5):715-23. doi: 10.1089/gtmb.2010.0087. Epub 2010 Sep 20.
Widespread use of tobacco and betel quid consumption and a high incidence of tobacco-associated aerodigestive tract cancers have been reported in different ethnic groups from several regions of Northeast (NE) India. This study was done to explore the possibility of phase II metabolic enzymes being responsible for the high prevalence of cancers in this region of India.
Samples from 370 cases with oral, gastric, and lung cancers and 270 controls were analyzed for polymorphism of glutathione-S-transferase (GST) genes using polymerase chain reaction-restriction fragment length polymorphism-based methods.
Tobacco smoking and betel quid chewing were found to be high risk factors for oral and lung cancers but not for gastric cancer, whereas tobacco chewing was found to be a risk factor for oral cancer but not for gastric or lung cancer. The variant genotypes of GSTP1 were not associated with any of the aerodigestive tract cancers. GSTT1 and GSTM1 null genotypes appeared to play a protective role for lung cancer (odds ratio [OR] = 0.47, 95% confidence interval [95% CI]: 0.24-0.93, p = 0.03) and (OR = 0.52, 95% CI: 0.28-0.96, p = 0.04), but they were not associated with oral and gastric cancers. However, when data was analyzed in different geographic regions the GSTT1 null genotype was found to be a significant risk factor for oral (OR = 2.58, 95% CI 1.01-6.61, p = 0.05) as well as gastric cancer (OR = 3.08, 95% CI 1.32-7.19, p = 0.009) in samples obtained from the Assam region of NE India. This is the first study on the association of GST polymorphisms and aerodigestive tract cancers in the high-risk region of NE India.
据报道，印度东北部（NE）多个地区的不同种族群体中，烟草广泛使用、槟榔咀嚼现象普遍，且与烟草相关的上消化道癌症发病率很高。本研究旨在探讨II期代谢酶是否是导致该地区癌症高发的原因。
采用基于聚合酶链反应-限制性片段长度多态性的方法，对370例口腔癌、胃癌和肺癌患者及270例对照的样本进行谷胱甘肽-S-转移酶（GST）基因多态性分析。
吸烟和嚼槟榔被发现是口腔癌和肺癌的高危因素，但不是胃癌的高危因素，而嚼烟草被发现是口腔癌的危险因素，但不是胃癌或肺癌的危险因素。GSTP1的变异基因型与任何上消化道癌症均无关联。GSTT1和GSTM1缺失基因型似乎对肺癌具有保护作用（比值比[OR]=0.47，95%置信区间[95%CI]：0.24-0.93，p=0.03）和（OR=0.52，95%CI：0.28-0.96，p=0.04），但它们与口腔癌和胃癌无关。然而，当在不同地理区域分析数据时，发现GSTT1缺失基因型是印度东北部阿萨姆地区样本中口腔癌（OR=2.58，95%CI 1.01-6.61，p=0.05）以及胃癌（OR=3.08，95%CI 1.32-7.19，p=0.009）的显著危险因素。这是印度东北部高危地区关于GST多态性与上消化道癌症关联研究的首次报道。
