Li Shao-bin, Yan Yu-sheng, Li Hui, Chen Kun-tang, Tong Jian, Chen Qun-qing, Zhang Fu-wei, Xiao Fei
Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Sep;30(9):2100-3.
To evaluate the hemocompatibility of a small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma treatment.
The composite blood vessel was prepared by first coating the small-caliber expanded polytetrafluoroethylene vessel with silk fibroin followed by sulfonation by low temperature plasma treatment. After hemolysis test in vitro, dynamic coagulation time test, blood platelet adhesion test, and recalcification time test were performed to evaluate the hemocompatibility of the composite blood vessel.
Scanning electronic microscopy revealed obvious platelets adhesion on the conventional artificial (control) vessel, which seldom occurred on the composite vessel. The curve of absorbance-clotting time of the composite vessel declined more slowly than that of the control vessel. The recalcification time of the composite blood vessel averaged 603 s, significantly longer than that of the control vessel (480 s, P = 0.000).
The composite blood vessel has good antithrombotic activity and hemocompatibility as a promising vascular prosthesis.
评估经低温等离子体处理磺化的丝素蛋白涂层小口径膨体聚四氟乙烯血管的血液相容性。
通过先在小口径膨体聚四氟乙烯血管上涂覆丝素蛋白，然后进行低温等离子体处理磺化来制备复合血管。体外溶血试验后，进行动态凝血时间试验、血小板黏附试验和复钙时间试验，以评估复合血管的血液相容性。
扫描电子显微镜显示传统人工（对照）血管上有明显的血小板黏附，而在复合血管上很少发生。复合血管的吸光度 - 凝血时间曲线下降比对照血管更慢。复合血管的复钙时间平均为603秒，明显长于对照血管（480秒，P = 0.000）。
作为一种有前景的血管假体，复合血管具有良好的抗血栓活性和血液相容性。
