Bui Hieu T, Friederich Aidan Rw, Li Emily, Prawel David A, James Susan P
1 School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USA.
2 Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, USA.
J Biomater Appl. 2018 Jul;33(1):52-63. doi: 10.1177/0885328218776807. Epub 2018 May 28.
Heart disease continues to be the leading cause of death in the United States. The demand for cardiovascular bypass procedures increases annually. Expanded polytetrafluoroethylene is a popular material for replacement implants, but it does have drawbacks such as high thrombogenicity and low patency, particularly in small diameter grafts. Hyaluronan, a naturally occurring polysaccharide in the human body, is known for its wound healing and anticoagulant properties. In this work, we demonstrate that treating the luminal surface of expanded polytetrafluoroethylene grafts with hyaluronan improves hemocompatibility without notably changing its mechanical properties and without significant cytotoxic effects. Surface characterization such as ATR-FTIR and contact angle goniometry demonstrates that hyaluronan treatment successfully changes the surface chemistry and increases hydrophilicity. Tensile properties such as elastic modulus, tensile strength, yield stress and ultimate strain are unchanged by hyaluronan enhancement. Durability data from flow loop studies demonstrate that hyaluronan is durable on the expanded polytetrafluoroethylene inner lumen. Hemocompatibility tests reveal that hyaluronan-treated expanded polytetrafluoroethylene reduces blood clotting and platelet activation. Together our results indicate that hyaluronan-enhanced expanded polytetrafluoroethylene is a promising candidate material for cardiovascular grafts.
心脏病仍然是美国的主要死因。心血管搭桥手术的需求每年都在增加。膨体聚四氟乙烯是一种用于替代植入物的常用材料，但它确实存在一些缺点，如高血栓形成性和低通畅率，特别是在小直径移植物中。透明质酸是人体内天然存在的一种多糖，以其伤口愈合和抗凝特性而闻名。在这项工作中，我们证明用透明质酸处理膨体聚四氟乙烯移植物的管腔表面可改善血液相容性，而不会显著改变其机械性能，也不会产生明显的细胞毒性作用。诸如衰减全反射傅里叶变换红外光谱（ATR-FTIR）和接触角测量等表面表征表明，透明质酸处理成功改变了表面化学性质并增加了亲水性。透明质酸增强后，诸如弹性模量、拉伸强度、屈服应力和极限应变等拉伸性能并未改变。流动环路研究的耐久性数据表明，透明质酸在膨体聚四氟乙烯内表面上具有耐久性。血液相容性测试表明，经透明质酸处理的膨体聚四氟乙烯可减少血液凝固和血小板活化。我们的结果共同表明，透明质酸增强的膨体聚四氟乙烯是一种有前途的心血管移植物候选材料。
