Chen Wei-yin, Sun Cheng-ming, Wang Hui-ming, Huang Mei, Zhu Guan-xiang, Zhu Bi-de, Liu Fu-you
Department of nurology, Affiliated Hospital, ChengDu University of TCM, ChengDu University of TCM, Chendu 610072, China. weiyinchen
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Sep;30(9):2115-8.
To observe the effect of tanshinone IIA (TS IIA) pretreatment on the expression of the inflammatory factor IL-1β and RelA mRNA in rats with focal cerebral ischemia.
A total of 100 adult male SD rats were randomly divided into 6 groups, namely the model, ischemic preconditioning (IPC), TSIIA preconditioning, TSIIA treatment, sham-operated, and blank control groups. In the former 4 groups, rat models of focal cerebral ischemia were established with corresponding treatments. The expressions of IL-1β and RelA mRNA in each group were detected using RT-PCR.
All the groups showed expressions of IL-1β and RelA mRNA with the exception of the blank control group. Compared to the model group, TSIIA preconditioning group, TSIIA treatment group, and IPC group all had significantly reduced expression of IL-1β and RelA mRNA (P < 0.05). The expressions were lower in IPC group than in TSIIA preconditioning group and TSIIA treatment group(P < 0.05), and no significant difference was found in the expressions between the latter two groups.
The protective effect of pretreatment with TS IIA against cerebral ischemia is related to the reduction of IL-1β and RelA mRNA expressions.
观察丹参酮IIA（TS IIA）预处理对局灶性脑缺血大鼠炎症因子白细胞介素-1β（IL-1β）和RelA mRNA表达的影响。
将100只成年雄性SD大鼠随机分为6组，即模型组、缺血预处理（IPC）组、TSIIA预处理组、TSIIA治疗组、假手术组和空白对照组。前4组采用相应处理建立局灶性脑缺血大鼠模型。采用逆转录聚合酶链反应（RT-PCR）检测各组IL-1β和RelA mRNA的表达。
除空白对照组外，其余各组均有IL-1β和RelA mRNA表达。与模型组相比，TSIIA预处理组、TSIIA治疗组和IPC组IL-1β和RelA mRNA表达均显著降低（P<0.05）。IPC组表达低于TSIIA预处理组和TSIIA治疗组（P<0.05），后两组之间表达无显著差异。
TS IIA预处理对脑缺血的保护作用与降低IL-1β和RelA mRNA表达有关。
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