Johnson M L, Lassiter A E
Department of Pharmacology, University of Virginia Health Sciences Center, Charlottesville 22908.
Biophys Chem. 1990 Oct;38(1-2):159-65. doi: 10.1016/0301-4622(90)80051-8.
One group of laboratories uses an 'even-weighted', or unweighted, nonlinear least-squares method for the analysis of experimental oxygen binding data obtained with an Imai oxygenation apparatus. Another group uses an 'end-weighted' nonlinear least-squares analysis. With end weighting each observation is assigned a statistical weight which is proportional to Y(1-Y), where Y is the fractional saturation. In this work we discuss statistical weighting functions as applied to the Imai oxygenation apparatus and then determined what are the best weighting factors for an actual series of published experiments. Based on these calculations, it is concluded that the 'best' weighting for the Imai oxygenation apparatus is a very small amount of end weighting. Furthermore, the amount of end weighting is so small that even weighting, or no weighting at all, is also appropriate.
一组实验室使用“等权重”或未加权的非线性最小二乘法来分析使用今井氧合装置获得的实验氧结合数据。另一组使用“末端加权”非线性最小二乘分析。在末端加权中，每个观测值被赋予一个与Y(1 - Y)成比例的统计权重，其中Y是分数饱和度。在这项工作中，我们讨论了应用于今井氧合装置的统计加权函数，然后确定了一系列实际已发表实验的最佳加权因子。基于这些计算，得出的结论是，今井氧合装置的“最佳”加权是非常少量的末端加权。此外，末端加权的量非常小，以至于等权重或根本不加权也是合适的。
