使用中空微针将药物递送至眼后脉络膜。
Suprachoroidal drug delivery to the back of the eye using hollow microneedles.
机构信息
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, Georgia 30332-0100, USA.
出版信息
Pharm Res. 2011 Jan;28(1):166-76. doi: 10.1007/s11095-010-0271-y. Epub 2010 Sep 21.
PURPOSE
In this work, we tested the hypothesis that microneedles provide a minimally invasive method to inject particles into the suprachoroidal space for drug delivery to the back of the eye.
METHODS
A single, hollow microneedle was inserted into the sclera, and infused nanoparticle and microparticle suspensions into the suprachoroidal space. Experiments were performed on whole rabbit, pig, and human eyes ex vivo. Particle delivery was imaged using brightfield and fluorescence microscopy as well as microcomputed tomography.
RESULTS
Microneedles were shown to deliver sulforhodamine B as well as nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig, and human eyes. Volumes up to 35 μL were administered consistently. Optimization of the delivery device parameters showed that microneedle length, pressure, and particle size played an important role in determining successful delivery into the suprachoroidal space. Needle lengths of 800-1,000 μm and applied pressures of 250-300 kPa provided most reliable delivery.
CONCLUSIONS
Microneedles were shown for the first time to deliver nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig and human eyes. This shows that microneedles may provide a minimally invasive method for controlled drug delivery to the back of the eye.
目的
在这项工作中，我们检验了这样一个假设，即微针提供了一种微创方法将颗粒注入脉络膜上腔，以便将药物递送到眼睛后部。
方法
将单个空心微针插入巩膜，并将纳米颗粒和微颗粒悬浮液注入脉络膜上腔。在整个兔、猪和人眼球的离体实验中进行了实验。使用明场和荧光显微镜以及微计算机断层扫描来对颗粒递送来进行成像。
结果
微针能够将磺基罗丹明 B 以及纳米颗粒和微颗粒悬浮液递送到兔、猪和人眼球的脉络膜上腔。一致地施予了高达 35 μL 的体积。对递药装置参数的优化表明，微针长度、压力和颗粒大小对成功递送到脉络膜上腔起着重要作用。800-1000 μm 的针长和 250-300 kPa 的应用压力提供了最可靠的递药。
结论
首次表明微针可将纳米颗粒和微颗粒悬浮液递送到兔、猪和人眼球的脉络膜上腔。这表明微针可能为眼睛后部的药物控制递供提供了一种微创方法。
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