Experimental Orthopaedics, Department of Clinical and Experimental Medicine, Faculty of Medicine, Linköping University, 58183 Linköping, Sweden.
J Mater Sci Mater Med. 2010 Nov;21(11):3029-37. doi: 10.1007/s10856-010-4154-x. Epub 2010 Sep 21.
Previous studies show that surface immobilized bisphosphonates improve the fixation of stainless steel screws in rat tibia after 2-8 weeks of implantation. We report here about the immobilization of a potent bisphosphonate, zoledronate, to crosslinked fibrinogen by the use of another technique, i.e. ethyl-dimethyl-aminopropylcarbodiimide (EDC)/imidazole immobilization. Bone fixation of zoledronate-coated screws was compared to screws coated with crosslinked fibrinogen only and ditto with EDC/N-hydroxy-succinimide immobilized pamidronate. Fixation in rat tibia was evaluated by a pull-out test at either 2 or 6 weeks after implantation. Both bisphosphonate coatings increased the pull-out force at both time points, and zoledronate showed a significantly higher pull-out force than pamidronate. To further evaluate the new coating technique we also performed a morphometric study, focusing on the area surrounding the implant. The zoledronate coating resulted in an increased bone density around the screws compared to controls. No pronounced increase was seen around the pamidronate coated screws. Together, the results demonstrate the possibility of obtaining a significant local therapeutic effect with minute amounts of surface immobilized zoledronate.
先前的研究表明，表面固定的双膦酸盐可改善不锈钢螺钉在大鼠胫骨内植入 2-8 周后的固定效果。我们在此报告了一种强效双膦酸盐唑来膦酸通过另一种技术，即乙基二甲氨基丙基碳化二亚胺（EDC）/咪唑固定交联纤维蛋白原。唑来膦酸涂层螺钉的骨固定效果与仅用交联纤维蛋白原涂层的螺钉以及用 EDC/N-羟基琥珀酰亚胺固定帕米膦酸的螺钉进行了比较。在植入后 2 或 6 周时通过拔出试验评估固定情况。两种双膦酸盐涂层均在两个时间点增加了拔出力，且唑来膦酸的拔出力明显高于帕米膦酸。为了进一步评估新的涂层技术，我们还进行了形态计量学研究，重点关注植入物周围的区域。与对照组相比，唑来膦酸涂层导致螺钉周围的骨密度增加。在帕米膦酸涂层的螺钉周围未见明显增加。总之，这些结果表明，用少量表面固定的唑来膦酸可以获得显著的局部治疗效果。
