Vegas Arturo J, Koehler Angela N
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Methods Mol Biol. 2010;669:43-55. doi: 10.1007/978-1-60761-845-4_4.
General binding assays involving microarrays of small molecules can be used to identify small molecule ligands for nearly any protein, even in the absence of knowledge about protein structure or function. Several suitable methods for manufacturing small molecule microarrays (SMMs) exist and different immobilization methods may be more or less preferable for any given application. Here, we describe a protocol for noncovalent and homogenous capture of small molecules using fluorous interactions between small molecules containing fluorocarbon tags and fluorocarbon-coated glass surfaces. These arrays are especially useful for applications that require display of compounds in a specific orientation such as screening biased libraries.
涉及小分子微阵列的一般结合测定可用于识别几乎任何蛋白质的小分子配体，即使在对蛋白质结构或功能一无所知的情况下也能做到。目前存在几种制造小分子微阵列（SMM）的合适方法，对于任何给定的应用，不同的固定方法可能或多或少更可取。在这里，我们描述了一种使用含氟碳标签的小分子与氟碳涂层玻璃表面之间的氟相互作用进行小分子非共价和均匀捕获的方案。这些阵列对于需要以特定方向展示化合物的应用特别有用，例如筛选偏向文库。
