Department of Chemistry and The Plant Sciences Institute, Iowa State University, 2756 Gilman, Ames, IA 50011, USA.
Future Med Chem. 2009 Aug;1(5):889-96. doi: 10.4155/fmc.09.76.
Microarray techniques based on covalent and noncovalent compound immobilization have been developed for screening proteins, antibodies and enzymes to probe the possible biological roles of these interactions as well as their therapeutic and diagnostic potential. Small-molecule microarrays are particularly valuable for creating and probing multivalent displays of molecules, such as saccharides that mimic the multivalent displays of cell surface-bound compounds. After development of the method for the screening of carbohydrates in a multivalent display format, microarrays based on noncovalent fluorous interactions have seen use in probing protein-binding partners with a range of arrayed compounds. In this article, existing strategies and future perspectives for fluorous-based small-molecule microarrays for protein, antibody and enzyme screening will be presented. To date, qualitative and quantitative fluorous-based microarrays have offered important information regarding biomolecular interactions. Larger compound arrays created with automated fluorous-based synthesis and diagnostic tools based on fluorous-based microarrays are likely ahead.
基于共价和非共价化合物固定化的微阵列技术已被开发出来，用于筛选蛋白质、抗体和酶，以探究这些相互作用的可能生物学作用及其治疗和诊断潜力。小分子微阵列对于创建和探测分子的多价展示特别有价值，例如模拟细胞表面结合化合物的多价展示的糖。在开发用于筛选多价展示形式中的碳水化合物的方法之后，基于非共价氟相互作用的微阵列已用于探测与一系列阵列化合物结合的蛋白质结合伴侣。在本文中，将介绍用于筛选蛋白质、抗体和酶的基于氟的小分子微阵列的现有策略和未来展望。迄今为止，基于氟的定性和定量微阵列已经提供了关于生物分子相互作用的重要信息。使用自动化基于氟的合成创建更大的化合物阵列，并使用基于氟的微阵列作为诊断工具，有望取得更大的进展。
