School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
J Cell Physiol. 2011 Apr;226(4):1069-73. doi: 10.1002/jcp.22429.
Store-operated calcium entry (SOCE) is essential for many cellular processes. In this study, we investigated modulation of SOCE by tyrosine phosphorylation in rat epididymal basal cells. The intracellular Ca(2+) ([Ca(2+)]i) measurement showed that SOCE occurred in rat epididymal basal cells by pretreating the cells with thapsigargin (Tg), the inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPase. To identify the role of Ca(2+) channels in this response, we examined the effects of transient receptor potential canonical channel blockers 2-aminoethoxydiphenyl borate (2-APB), 1-[β-[3-(4-methoxyphenyl)pro-poxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride(SKF96365), Gd(3+), and non-selective cation channel blocker Ni(2+) respectively on SOCE and found that these blockers could inhibit the Ca(2+) influx to different extent. Furthermore, we studied the regulation of SOCE by tyrosine kinase pathway. The inhibitor of tyrosine kinase genistein remarkably suppressed the SOCE response, whereas sodium orthovanadate, the inhibitor of tyrosine phosphatase, greatly enhanced it. The results suggest that tyrosine kinase pathway plays a significant role in the initiation of SOCE and positively modulates SOCE in epididymal basal cells.
钙库操纵性钙内流（SOCE）是许多细胞过程所必需的。在这项研究中，我们研究了酪氨酸磷酸化对大鼠附睾基底细胞 SOCE 的调节作用。细胞内 Ca(2+)([Ca(2+)]i)测量表明，通过用肌浆内质网 Ca(2+)-ATP 酶抑制剂 thapsigargin (Tg)预处理细胞，大鼠附睾基底细胞中发生了 SOCE。为了确定 Ca(2+)通道在这种反应中的作用，我们研究了瞬时受体电位经典通道阻滞剂 2-氨基乙氧基二苯硼酸盐 (2-APB)、1-[β-[3-(4-甲氧基苯基)丙氧基]-4-甲氧基苯乙基]-1H-咪唑盐酸盐(SKF96365)、Gd(3+)和非选择性阳离子通道阻滞剂 Ni(2+)对 SOCE 的影响，发现这些阻滞剂可以不同程度地抑制 Ca(2+)内流。此外，我们研究了酪氨酸激酶途径对 SOCE 的调节作用。酪氨酸激酶抑制剂金雀异黄素显著抑制 SOCE 反应，而酪氨酸磷酸酶抑制剂偏钒酸钠则大大增强了 SOCE 反应。结果表明，酪氨酸激酶途径在 SOCE 的启动中起重要作用，并正向调节附睾基底细胞中的 SOCE。
