USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX 78028, USA.
J Econ Entomol. 2010 Aug;103(4):1360-4. doi: 10.1603/ec09329.
Synergistic effects of verbutin, a member of aryl alkynyl derivatives, to three commonly used acaricides were evaluated with the modified Food and Agricultural Organization Larval Packet Test (FAO-LPT) against both susceptible and resistant strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae). These tick strains demonstrated various levels of resistance to coumaphos (2.5-8.2x), permethrin (57.9-711.7x), and amitraz (3.5-177.5x). Verbutin alone was more toxic to tick larvae than piperonyl butoxide (PBO), a conventional pesticide synergist. With synergism ratios (SRs) ranging from 1.5 to 6.0, verbutin was more potent than PBO (SR = 0.9-1.6) in synergizing coumaphos toxicity to resistant tick larvae. Similarly, verbutin (SR = 1.8-15) was also found to be more potent than PBO (SR = 0.9-2.5) in synergizing amitraz in resistant tick strains. Both verbutin and PBO significantly increased permethrin toxicity to larvae of all tick strains tested, and there was no significant difference between the two synergists. SRs ranged from 2.1 to 4.4 and from 2.1 to 3.6 for PBO and verbutin, respectively.
用改良的粮农组织幼虫包检法（FAO-LPT），评估了苯乙炔基衍生物成员之一的 Verbutin 与三种常用杀蜱剂对南方牛蜱（Rhipicephalus（Boophilus）microplus（Canestrini））敏感和抗性品系的协同作用（蜱螨目：硬蜱科）。这些蜱虫品系对氯菊酯（2.5-8.2x）、氯氰菊酯（57.9-711.7x）和甲氰菊酯（3.5-177.5x）表现出不同程度的抗性。Verbutin 对蜱幼虫的毒性比传统农药增效剂胡椒基丁醚（PBO）更强。协同作用比值（SR）范围为 1.5 至 6.0，Verbutin 比 PBO（SR=0.9-1.6）更能增强对抗性蜱幼虫的氯菊酯毒性协同作用。同样，Verbutin（SR=1.8-15）也比 PBO（SR=0.9-2.5）更能增强抗性蜱株中甲氰菊酯的增效作用。Verbutin 和 PBO 均显著增加了所有测试蜱株幼虫对氯氰菊酯的毒性，两种增效剂之间没有显著差异。SR 范围分别为 2.1 至 4.4 和 2.1 至 3.6，分别为 PBO 和 Verbutin。
