Department of Clinical Biochemistry, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey.
Brain Res Bull. 2010 Nov 20;83(6):356-9. doi: 10.1016/j.brainresbull.2010.09.007. Epub 2010 Sep 19.
the aim of our study was to evaluate the activity of superoxide dismutase (SOD) and the levels of glutathione (GSH), malondialdehyde (MDA), and nitric oxide (NO) in liver and kidney tissues in a rat model of convulsive seizure induced by single and repeated doses of pentylenetetrazole (PTZ) and sound stimulation with key ringing.
male Wistar adult rats (n=48), were used in the experiment. The animals were divided into six groups: (1) Single Seizure Control Group (SS-Control; n=8), (2) Repeated Seizures Control Group (RS-Control; n=8), (3) PTZ induced Single Seizure Group (SS-PTZ Group; n=8), (4) PTZ induced Repeated Seizures Group (RS- PTZ Group; n=8), (5) Key-Ringing Induced Single Seizure Group (SS-KEY Group; n=8), (6) Key-Ringing Induced Repeated Seizures Group (RS-KEY Group; n=8). Following injections rats were observed for seizure activity for 30 min. Animals were sacrificed 24h after induced seizure (single or last seizure) or saline administration. MDA, NO, GSH levels and SOD activities were determined in liver and kidney tissues.
there was no significant difference between SS-Control and RS-Control groups, SS-PTZ and SS-KEY groups, and RS-PTZ and RS-KEY groups (p>0.05) in none of the examined 4 parameters in liver and kidney tissues. The liver and kidney levels of MDA and NO in SS-PTZ group were found to be significantly higher than the SS-Control group (p<0.05). In SS-KEY group, the liver and kidney levels of MDA and NO were found to be significantly higher and GSH levels were significantly lower than the SS-Control group (p<0.05). While liver and kidney levels of MDA in RS-PTZ group and RS-KEY group were found to be significantly higher than the RS-Control group (p<0.05), liver and kidney GSH levels were significantly lower (p<0.05). The liver levels of NO in RS-PTZ group and RS-KEY group were found to be significantly higher than the RS-Control group (p<0.05). Kidney SOD activities in RS-PTZ group and RS-KEY group were found to be significantly lower than the RS-Control group (p<0.05). When RS-PTZ group is compared with the SS-PTZ group, the liver SOD activity and kidney NO level were found to be significantly lower in the RS-PTZ group (p<0.05). While the liver NO level and GSH level in RS-KEY group were significantly higher than the SS-KEY group, SOD activity was significantly lower in the RS-KEY group (p<0.05). When RS-KEY group was compared with SS-KEY group, the kidney NO level and SOD activity were found to be significantly lower in the RS-KEY group (p<0.05).
in conclusion, key-ringing or PTZ induced single and repeated seizures result in increased oxidative damage and lipid peroxidation, and decreased antioxidant defense mechanisms.
本研究旨在评估单次和重复戊四氮（PTZ）和钥匙环声刺激诱导的癫痫发作后，大鼠肝、肾组织中超氧化物歧化酶（SOD）活性、谷胱甘肽（GSH）水平、丙二醛（MDA）和一氧化氮（NO）水平。
雄性 Wistar 成年大鼠（n=48）用于实验。动物分为六组：（1）单次惊厥对照（SS-Control）组（n=8）；（2）重复惊厥对照（RS-Control）组（n=8）；（3）PTZ 诱导的单次惊厥（SS-PTZ）组（n=8）；（4）PTZ 诱导的重复惊厥（RS-PTZ）组（n=8）；（5）钥匙环诱导的单次惊厥（SS-KEY）组（n=8）；（6）钥匙环诱导的重复惊厥（RS-KEY）组（n=8）。注射后，观察大鼠 30 分钟的惊厥活动。在诱导惊厥（单次或最后一次）或生理盐水给药后 24 小时处死动物。测定肝、肾组织中 MDA、NO、GSH 水平和 SOD 活性。
在肝、肾组织的 4 个检测参数中，SS-Control 组和 RS-Control 组、SS-PTZ 组和 SS-KEY 组、RS-PTZ 组和 RS-KEY 组之间无显著差异（p>0.05）。SS-PTZ 组的肝、肾组织 MDA 和 NO 水平明显高于 SS-Control 组（p<0.05）。SS-KEY 组肝、肾组织 MDA 和 NO 水平明显升高，GSH 水平明显降低（p<0.05）。RS-PTZ 组和 RS-KEY 组肝、肾组织 MDA 水平明显高于 RS-Control 组（p<0.05），肝、肾组织 GSH 水平明显降低（p<0.05）。RS-PTZ 组和 RS-KEY 组肝组织 NO 水平明显高于 RS-Control 组（p<0.05）。RS-PTZ 组和 RS-KEY 组肾组织 SOD 活性明显低于 RS-Control 组（p<0.05）。与 SS-PTZ 组相比，RS-PTZ 组肝 SOD 活性和肾 NO 水平明显降低（p<0.05）。RS-KEY 组肝组织 NO 水平和 GSH 水平明显高于 SS-KEY 组，SOD 活性明显降低（p<0.05）。与 SS-KEY 组相比，RS-KEY 组肾组织 NO 水平和 SOD 活性明显降低（p<0.05）。
总之，钥匙环或 PTZ 诱导的单次和重复惊厥导致氧化损伤和脂质过氧化增加，抗氧化防御机制降低。
