Institute of Biochemistry and Molecular Biology, Shandong University School of Medicine, 44# Wenhua Xi Road, Jinan, 250012 Shandong, People's Republic of China.
Mol Biol Rep. 2011 Mar;38(3):1869-74. doi: 10.1007/s11033-010-0304-y. Epub 2010 Sep 22.
Zinc is an essential nutrient for all organisms, which is involved in the function of numerous key enzymes in metabolism. Two gene families have been identified involved in zinc homeostasis. ZnT transporters reduce intracellular zinc while Zip transporters increase intracellular zinc. Previous studies in our laboratory have shown that Zip-1, ZnT-1, Zip-2 and LIV-1 mRNA are associated with zinc level in established human breast cancer in nude mice model. In this study, six zinc transporters: ZnT-1, ZnT-2, ZnT-4, Zip-1, Zip-8 and Zip-13 were chosen. We aim to determine the relation between zinc transporters and zinc level in kidney and lung of Wistar rats. Eighteen Wistar rats were randomly divided into three groups: normal group, zinc-deficiency group and pair-fed group. After 22 days, the rats were killed and organs samples were taken, then zinc transporters mRNA were detected by RT-PCR. Compared with the normal group, Zip-13 shows an up-regulation (P < 0.05) in zinc-deficiency group both in kidney and lung, and Zip-8 was significantly lower (P < 0.05) in zinc-deficiency group in kidney.
锌是所有生物体必需的营养物质，参与代谢中许多关键酶的功能。已经确定了两个与锌稳态相关的基因家族。ZnT 转运蛋白降低细胞内锌含量，而 Zip 转运蛋白增加细胞内锌含量。我们实验室之前的研究表明，Zip-1、ZnT-1、Zip-2 和 LIV-1mRNA 与裸鼠模型中已建立的人乳腺癌中的锌水平相关。在这项研究中，选择了六种锌转运蛋白：ZnT-1、ZnT-2、ZnT-4、Zip-1、Zip-8 和 Zip-13。我们旨在确定肾脏和肺组织中锌转运蛋白与锌水平之间的关系。18 只 Wistar 大鼠随机分为三组：正常组、缺锌组和配对喂养组。22 天后，处死大鼠并采集器官样本，然后通过 RT-PCR 检测锌转运蛋白 mRNA。与正常组相比，缺锌组肾脏和肺部的 Zip-13 表达上调（P<0.05），而肾脏中 Zip-8 的表达显著降低（P<0.05）。
