Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, Split.
Chem Biodivers. 2010 Sep;7(9):2309-25. doi: 10.1002/cbdv.201000021.
Salix spp. nectar honey volatiles of Croatian origin were analyzed by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE), followed by gas chromatography and mass spectrometry (GC, GC/MS). Isolated volatiles were found in the honey headspace and extracts with almost exclusive distribution of several abundant compounds (e.g., phenylacetic acid, pinocembrin, 8-hydroxy-4,7-dimethylcoumarin, and 3-hydroxy-trans-β-damascone in the extracts, or safranal and lilac alcohols in the headspace). Comparison with Croatian Salix spp. honeydew honey revealed similarities regarding distribution of important shikimate pathway derivatives (e.g., high percentage of phenylacetic acid) and several norisoprenoids (α-isophorone and 4-oxoisophorone). On the other hand, distinct features of this honey were occurrence of compounds such as pinocembrin, 8-hydroxy-4,7-dimethylcoumarin, phenylacetonitrile, norisoprenoids (major ones: 3-hydroxy-trans-β-damascenone and trans-β-damascone), more pronounced variability of linalool-derived compounds, as well as the abundance of 3-methylpropanoic acid, 3-methylbutanoic acid, 2-methylpentanoic acid, and 3-methylpentan-1-ol.
用顶空固相微萃取（HS-SPME）和超声溶剂萃取（USE）分析了源自克罗地亚的柳属花蜜挥发性物质，然后进行气相色谱和质谱分析（GC、GC/MS）。在蜂蜜的顶空和提取物中分离出了挥发性物质，其中几种丰富的化合物（例如，苯乙酸、松属素、8-羟基-4,7-二甲基香豆素和 3-羟基-trans-β-大马酮在提取物中，或香柠檬醛和紫丁香醇在顶空）几乎呈独占分布。与克罗地亚柳属蜜露蜂蜜的比较表明，重要的莽草酸途径衍生物（例如，苯乙酸的高百分比）和几种法呢烯（α-异佛尔酮和 4-氧异佛尔酮）的分布存在相似性。另一方面，这种蜂蜜的独特之处在于存在一些化合物，如松属素、8-羟基-4,7-二甲基香豆素、苯乙腈、法呢烯（主要的有：3-羟基-trans-β-大马酮和反式-β-大马酮）、更为明显的芳樟醇衍生化合物的可变性，以及 3-甲基丙酸、3-甲基丁酸、2-甲基戊酸和 3-甲基戊醇的丰富性。
