Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Cytotherapy. 2011 Mar;13(3):341-9. doi: 10.3109/14653249.2010.520312. Epub 2010 Sep 22.
Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation.
AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted.
eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability.
Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.
羊水是干细胞的已知来源。然而，尚未有关于马羊水干细胞的报道。我们已经分离出马羊水来源的多能干细胞（MSC）（eAF-MSC），并表明这些细胞具有自我更新能力和多系分化能力。
从纯种母马中获得羊水，并通过 Ficoll-Paque 密度梯度分离出单核细胞（MNC）。我们测量了累积倍增水平（CPDL），并通过流式细胞术对免疫表型进行了表征。为了研究分化能力，进行了三系分化测定。
可以分离出马羊水 MSC，且增殖水平较高。通过流式细胞术确定，eAF-MSC 呈现出典型的 MSC 表型标记。此外，eAF-MSC 显示出三系分化能力。
马羊水是 MSC 的良好来源。此外，eAF-MSC 可能可作为马的细胞治疗应用的有用材料。
