Tian Yunyun, Tao Li, Zhao Siriguleng, Tai Dapeng, Liu Dongjun, Liu Pengxia
College of Life Sciences, Inner Mongolia University, Inner Mongolia, Hohhot 010021, P.R.China.
Exp Anim. 2016 May 20;65(2):125-34. doi: 10.1538/expanim.15-0031. Epub 2015 Nov 27.
Mesenchymal stem cells (MSCs) are one of the most promising cell populations for tissue engineering and regenerative medicine. Of utmost importance to MSC research is identification of MSC sources that are easily obtainable and stable. Several studies have shown that MSCs can be isolated from amniotic fluid. The sheep is one of the main types of farm animal, and it has many biophysical and biochemical similarities to humans. Here, we obtained MSCs from ovine amniotic fluid and determined the expansion capacity, surface and intracellular marker expression, karyotype, and multilineage differentiation ability of these ovine amniotic fluid mesenchymal stem cells (oAF-MSCs). Moreover, expression levels of differentiation markers were measured using reverse transcription-qPCR (RT-qPCR). Our phenotypic analysis shows that the isolated oAF-MSCs are indeed MSCs.
间充质干细胞（MSCs）是组织工程和再生医学中最具前景的细胞群体之一。对于MSC研究而言，最重要的是鉴定易于获取且稳定的MSC来源。多项研究表明，MSCs可从羊水分离得到。绵羊是主要的家畜类型之一，与人类在许多生物物理和生化特性上具有相似性。在此，我们从绵羊羊水获取了MSCs，并测定了这些绵羊羊水间充质干细胞（oAF-MSCs）的扩增能力、表面和细胞内标志物表达、核型以及多向分化能力。此外，使用逆转录定量聚合酶链反应（RT-qPCR）测量分化标志物的表达水平。我们的表型分析表明，分离得到的oAF-MSCs确实是MSCs。
