Department of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria.
Clin Exp Allergy. 2010 Dec;40(12):1809-18. doi: 10.1111/j.1365-2222.2010.03602.x. Epub 2010 Sep 22.
Cow's milk is one of the most common causes of food allergy affecting approximately 2.5% of infants in the first years of their life. However, only limited information regarding the allergenic activity of individual cow's milk allergens is available.
To analyse the frequency of IgE reactivity and to determine the allergenic activity of individual cow's milk allergens.
A nitrocellulose-based microarray, based on purified natural and recombinant cow's milk allergens was used to determine IgE reactivity profiles using sera from 78 cow's milk-sensitized individuals of varying ages. The allergenic activity of the individual allergens was tested using patients' sera for loading rat basophil leukaemia cells (RBL) expressing the α-chain of the human receptor FcεRI.
Using the microarray and the RBL assay, cow's milk allergens were assessed for frequency of IgE recognition and allergenic activity. Moreover, the RBL assay allowed distinguishing individuals without or with mild clinical reactions from those with severe systemic or gastrointestinal symptoms as well as persons who grew out cow's milk allergy from those who did not.
Component-resolved testing using milk allergen microarrays and RBL assays seems to provide useful additional diagnostic information and may represent a basis for future forms of prophylactic and therapeutic strategies for cow's milk allergy.
牛奶是引起食物过敏的最常见原因之一，约影响生命最初几年内 2.5%的婴儿。然而，关于个体牛奶过敏原的变应原活性的信息十分有限。
分析 IgE 反应性的频率，并确定个体牛奶过敏原的变应原活性。
使用基于硝酸纤维素的微阵列，基于纯化的天然和重组牛奶过敏原，使用来自不同年龄的 78 个牛奶过敏个体的血清来确定 IgE 反应性图谱。使用表达人受体 FcεRIα链的载脂蛋白的患者血清来测试个体过敏原的变应原活性。
使用微阵列和 RBL 测定法，评估了牛奶过敏原的 IgE 识别频率和变应原活性。此外，RBL 测定法允许区分无或轻度临床反应的个体与有严重全身性或胃肠道症状的个体，以及从那些未能脱离牛奶过敏的个体中区分出已经脱离牛奶过敏的个体。
使用牛奶过敏原微阵列和 RBL 测定的成分分辨测试似乎提供了有用的额外诊断信息，并且可能为未来的牛奶过敏预防和治疗策略提供基础。
