Kumar Sushil, Chaudhary Swati, Sharma Vishakha, Kumari Renu, Mishra Raghvendra Kumar, Kumar Arvind, Choudhury Debjani Roy, Jha Ruchi, Priyadarshini Anupama, Kumar Arun
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110 067, India.
J Genet. 2010 Aug;89(2):201-11. doi: 10.1007/s12041-010-0026-x.
To understand the role of INSECATUS (INS) gene in pea, the leaf blades of wild-type, ins mutant and seven other genotypes, constructed by recombining ins with uni-tac, af, tl and mfp gene mutations, were quantitatively compared. The ins was inherited as a recessive mutant allele and expressed its phenotype in proximal leaflets of full size leaf blades. In ins leaflets, the midvein development was arrested in distal domain and a cleft was formed in lamina above this point. There was change in the identity of ins leaflets such that the intercalary interrupted midvein bore a leaf blade. Such adventitious blades in ins, ins tl and ins tl mfp were like the distal segment of respective main leaf blade. The ins phenotype was not seen in ins af and ins af uni-tac genotypes. There was epistasis of uni-tac over ins. The ins, tl and mfp mutations interacted synergistically to produce highly pronounced ins phenotype in the ins tl mfp triple mutant. The role(s) of INS in leaf-blade organogenesis are: positive regulation of vascular patterning in leaflets, repression of UNI activity in leaflet primordia for ectopic growth and in leaf-blade primordium for indeterminate growth of rachis, delimitation of proximal leaflet domain and together with TL and MFP homeostasis for meristematic activity in leaflet primordia. The variant apically bifid shape of the affected ins leaflets demonstrated that the leaflet shape is dependent on the venation pattern.
为了解豌豆中INSECATUS（INS）基因的作用，对野生型、ins突变体以及通过将ins与uni - tac、af、tl和mfp基因突变重组构建的其他七种基因型的叶片进行了定量比较。ins作为隐性突变等位基因遗传，并在全尺寸叶片的近端小叶中表现出其表型。在ins小叶中，中脉发育在远端区域停止，并且在该点上方的叶片中形成了一个裂缝。ins小叶的特征发生了变化，使得居间中断的中脉带有一个叶片。ins、ins tl和ins tl mfp中的这种不定叶片类似于各自主叶片的远端部分。在ins af和ins af uni - tac基因型中未观察到ins表型。uni - tac对ins存在上位性。ins、tl和mfp突变协同相互作用，在ins tl mfp三重突变体中产生高度明显的ins表型。INS在叶片器官发生中的作用是：正向调节小叶中的维管束模式，抑制小叶原基中异位生长的UNI活性以及叶片原基中轴的无限生长的UNI活性，界定近端小叶区域，并与TL和MFP一起维持小叶原基中的分生组织活性。受影响的ins小叶顶端二裂的形状表明小叶形状取决于叶脉模式。
