Kumar Sushil, Mishra Raghvendra Kumar, Kumar Anil, Srivastava Suchi, Chaudhary Swati
National Institute for Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, Post Box 10531, New Delhi, 110067, India.
Planta. 2009 Aug;230(3):449-58. doi: 10.1007/s00425-009-0952-0. Epub 2009 Jun 2.
Pisum sativum L., the garden pea crop plant, is serving as the unique model for genetic analyses of morphogenetic development of stipule, the lateral organ formed on either side of the junction of leafblade petiole and stem at nodes. The stipule reduced (st) and cochleata (coch) stipule mutations and afila (af), tendril-less (tl), multifoliate-pinna (mfp) and unifoliata-tendrilled acacia (uni-tac) leafblade mutations were variously combined and the recombinant genotypes were quantitatively phenotyped for stipule morphology at both vegetative and reproductive nodes. The observations suggest a role of master regulator to COCH in stipule development. COCH is essential for initiation, growth and development of stipule, represses the UNI-TAC, AF, TL and MFP led leafblade-like morphogenetic pathway for compound stipule and together with ST mediates the developmental pathway for peltate-shaped simple wild-type stipule. It is also shown that stipule is an autonomous lateral organ, like a leafblade and secondary inflorescence.
豌豆（Pisum sativum L.）作为一种园艺作物，是对托叶形态发生发育进行遗传分析的独特模型。托叶是在节处叶片叶柄与茎的连接处两侧形成的侧生器官。将托叶变小（st）和耳蜗状（coch）托叶突变以及无叶（af）、无卷须（tl）、多叶羽片（mfp）和单叶具卷须金合欢（uni-tac）叶片突变进行各种组合，并对重组基因型在营养节点和生殖节点的托叶形态进行定量表型分析。观察结果表明，主调控因子COCH在托叶发育中起作用。COCH对托叶的起始、生长和发育至关重要，抑制由UNI-TAC、AF、TL和MFP导致的复叶状托叶的叶片状形态发生途径，并与ST一起介导盾形简单野生型托叶的发育途径。研究还表明，托叶是一个自主的侧生器官，类似于叶片和二级花序。
