Tamura S, Kikuchi K, Hiraga A, Kikuchi H, Hosokawa M, Tsuiki S
Biochim Biophys Acta. 1978 Jun 9;524(2):349-56. doi: 10.1016/0005-2744(78)90171-7.
By using chromatography on DEAE-cellulose, aminohexyl-Sepharose 4B and Sephadex G-200, rat liver extract was shown to contain at least three fractions, IA, IB and II, of histone phosphatase. Fractions IA and II are probably the same enzymes as the previously described glycogen synthase phosphatase and phosphorylase phosphatase, respectively, but IB exhibits noticeable activities only with phosphohistone as substrate. Approximate molecular weights of 69 000, 300 000 and 160 000 were determined by gel filtration on Sephadex G-200 for IA, IB and II, respectively.
通过使用二乙氨基乙基纤维素（DEAE - 纤维素）、氨基己基琼脂糖4B和葡聚糖G - 200进行色谱分析，结果表明大鼠肝脏提取物中至少含有三种组蛋白磷酸酶组分，即IA、IB和II。组分IA和II可能分别与先前描述的糖原合酶磷酸酶和磷酸化酶磷酸酶是相同的酶，但IB仅以磷酸组蛋白为底物时表现出显著活性。通过在葡聚糖G - 200上进行凝胶过滤，分别测定出IA、IB和II的近似分子量为69000、300000和160000。
